天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (4): 360-363.doi: 10.11958/j.issn.0253-9896.2015.04.007

• 实验研究 • 上一篇    下一篇

酒精依赖对地西泮抗惊厥作用的影响

汤洁1，王朵朵1，马焦1，凤雅薇1，楼鹏1，张心宇1，徐佳晨1，高洋洋1，武静茹2△   

  1. 1徐州医学院麻醉学院2011级2班（邮编221000）；2徐州医学院麻醉生理学教研室
  • 收稿日期:2014-10-09 修回日期:2014-11-29 出版日期:2015-04-15 发布日期:2015-04-13
  • 通讯作者: 武静茹 E-mail:wujingr5810@sina.com
  • 作者简介:汤洁(1993)，女，本科在读，主要从事神经生理研究
  • 基金资助:
    国家自然科学基金青年基金项目（81200861）；徐州市科技计划项目（KC14SH075）；地方高校国家级大学生创新创业训练计划项目（201310313013）

Impacts of alcohol dependence on the anticonvulsant effect of diazepam

TANG Jie1, WANG Duoduo1, MA Jiao1, FENG Yawei1, LOU Peng1, ZHANG Xinyu1, XU Jiachen1, GAO Yangyang1, WU Jingru2△   

  1. 1 Class 2 , Grade 2011, School of Anesthesia, Xuzhou Medical College, Jiangsu 221000, China; 2 Teaching and Research Section of Anesthesia Physiology, Xuzhou Medical College
  • Received:2014-10-09 Revised:2014-11-29 Published:2015-04-15 Online:2015-04-13
  • Contact: WU Jingru E-mail:wujingr5810@sina.com

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摘要： 摘要：目的研究酒精依赖对地西泮抗惊厥作用的影响。方法成年昆明种小鼠36 只，均分为酒精依赖组（A 组）、地西泮组（D 组）和生理盐水组（N 组）。A 组用含0.8％乙醇的生理盐水进行腹腔注射，其他2 组注射生理盐水，每天上、下午各1 次，0.2 mL/次，注射7 d。检测小鼠7 d 的自主活动情况。7 d 后行电惊厥实验，A 组和D 组腹腔注射0.05%地西泮溶液0.05 mL/10 g，N 组注射等体积生理盐水，给药前及给药后15、30、60 min 分别测量3 组小鼠惊厥阈值。结果第2～6 天，A 组小鼠自主活动计数小于其他2 组（P＜0.05），第1 和7 天，3 组小鼠自主活动计数差异无统计学意义。给药前，A 组惊厥阈值高于D 组和N 组（P＜0.05）；给药后，N 组惊厥阈值与给药前相比差异无统计学意义；给药后15 min，D 组惊厥阈值高于A 组和N 组，A 组阈值高于N 组（P＜0.05）；给药后30 min、60 min，A 组和D 组惊厥阈值均高于N 组（P＜0.05），A 组和D 组惊厥阈值差异无统计学意义。结论酒精依赖有抗惊厥的作用；酒精依赖会使地西泮的抗惊厥作用减弱。

关键词: 酒精依赖；地西泮；抗惊厥；电刺激；&gamma, -氨基丁酸

Abstract: Abstract: Objective To study the impacts of alcohol dependence on the anticonvulsant effect of diazepam. Meth⁃ ods Kunming mice (n=36) were divided into 3 groups (n=12 in each group), Alcohol Dependence Group（A group）, Diaze⁃ pam Group（D group）and Normal Saline Group（N group）. A group received an intraperitoneal injection with a 0.2 mL dose of 0.8% alcohol in NS (normal saline) , while both D and N group received an injection with a 0.2 mL dose of NS without alco⁃ hol , twice a day. Mice’s autonomic activities were monitored every day. After 7 days, the electroconvulsive experiment was performed. Both A and D group were given a weight-based dose of 0.05 mL/10 g of 0.05% diazepam via intraperitoneal injection, while N group was given a 0.05 mL/10 g dose of NS. Before administration and after 15, 30, 60 min of administration, the convulsion threshold of each group was measured. Results The count of autonomic activity of mice in A group was less than that of mice in D and N group during the 2nd day to 6th day（P＜0.05）. On the 1st and 7th day, the difference of the count of autonomic activity of mice between A group and the other two groups was not statistically significant（P > 0.05）. The convulsion threshold of mice in A group was higher than that of mice in D and N group before administration（P＜0.05）. Af⁃ ter administration, the convulsion threshold of mice in N group didn’t show statistically significant difference from that of mice before administration（P > 0.05）. After 15 min of administration, the convulsion threshold of mice in D group was high⁃ er than that of mice in A and N group（P＜0.05）, while the convulsion threshold of mice in A group was higher than that of mice in N group（P＜0.05）. After 30 min and 60 min of administration, both the convulsion thresholds of mice in A and D group were higher than that of mice in N group（P＜0.05）. However, at this point, the difference of the convulsion thresholds of mice between A and D group was not statistically significant（P > 0.05）. Conclusion Alcohol dependence has anticon⁃ vulsant effect. Alcohol dependence weakens the anticonvulsant effect of diazepam.

Key words: alcohol dependence, diazepam, anticonvulsant action；electric stimulation；gamma-aminobutyric acid