天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (5): 488-490.doi: 10.11958/j.issn.0253-9896.2015.05.011

• 专题研究-缺血与再灌注损伤 • 上一篇    下一篇

PPARγ对大鼠心肌缺血/再灌注后恶性心律失常的影响

何学恕， 莫新玲△， 陈华， 谢婷#br# #br#   

  1. 桂林医学院附属医院心内科 （邮编541001）
  • 收稿日期:2014-10-28 修回日期:2014-12-17 出版日期:2015-05-15 发布日期:2015-05-25
  • 通讯作者: 莫新玲E-mail:glmxl1003@163.com E-mail:glmxl1003@163.com
  • 作者简介:何学恕 （1985）， 男， 硕士在读， 主要从事高血压、 冠心病的防治研究
  • 基金资助:
    广西壮族自治区卫生厅自筹课题 （Z2013492）； 广西壮族自治区卫生厅重点科研课题 （重2010049）

Effects of PPARγ on malignant arrhythmia in myocardial ischemia/reperfusion model rats

HE Xueshu, MO Xinling△, CHEN Hua, XIE Ting#br# #br#   

  1. Department of Cardiology, The Affiliated Hospital of Guilin Medical University, Guilin 541001, China
  • Received:2014-10-28 Revised:2014-12-17 Published:2015-05-15 Online:2015-05-25
  • Contact: MO Xinling,E-mail:glmxl1003@163.com E-mail:glmxl1003@163.com

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摘要： 摘要： 目的 探讨大鼠心肌过氧化物酶体增殖物激活受体（PPAR） γ对缺血/再灌注（I/R）后恶性心律失常的影响。方法 24 只 SD 大鼠随机分为 4 组： 假手术组（Sham 组）， 缺血/再灌注组（I/R 组）， 罗格列酮+缺血/再灌注组（ROS 组）， PPARγ抑制剂 GW9662+缺血/再灌注组 （GW 组） ,每组 6 只。采用冠状动脉左前降支结扎法制造 I/R 动物模型， 缺血 30 min， 再灌注 2 h。全程肢体Ⅱ导联心电图观察各组恶性心律失常发生次数并记录校正 QT 间期 （QTc）的变化， RT-PCR 检测 PPARγ mRNA 表达。结果 ROS 组发生 5 例恶性心律失常， I/R 组 2 例， GW 组 1 例， Sham 组 0 例。ROS 组的 QTc 间期在再灌注期较其他 3 组延长（均 P < 0.05）， 与 I/R 组和 ROS 组相比， GW 组的 QTc 间期在缺血 30 min 及再灌注过程中缩短（均 P < 0.05）。与 Sham 组相比， 其他 3 组 PPAR-γ mRNA 表达明显升高（均 P < 0.05）， ROS 组 PPARγ mRNA 表达水平最高， GW 组较 I/R 组和 ROS 组表达下调（均 P < 0.05）。结论 PPARγ过表达可能导致心肌 I/R 恶性心律失常的发生。

关键词: PPAR-γ, 心肌缺血/再灌注, 恶性心律失常, 罗格列酮

Abstract: Abstract: Objective To investigate the effects of peroxisome proliferator activated receptor gamma (PPARγ) on ma⁃ lignant arrhythmia in myocardial of ischemia/reperfusion(I/R) rats. Methods Twenty-four SD rats were randomly divided into four groups: Sham group, I/R group, rosiglitazone (ROS) group and PPARγ inhibitor GW9662 (GW) group. The myocar⁃ dial I/R injury was induced by ligation of the left anterior descending coronary artery, with ischemia for 30 min and reperfu⁃ sion for 2 h. The whole process limb Ⅱ lead electrocardiogram was applied to observe the frequency of malignant arrhythmia and record the corrected changes of QT(QTc) interval. RT-PCR was used to detect the expression of PPARγ mRNA. Re⁃ sults There were 5 cases of malignant arrhythmia in ROS group, 2 in I/R and 1 in GW group, 0 was found in Sham group. There was a prolongation of the QTc interval in ROS group than the other groups after ischemic stage (P < 0.05). Compared with I/R group and ROS group, the QTc intervals were shorten in ischemia 30 min and reperfusion process in GW group (P< 0.05). Compared with sham group, the expression of PPARγ mRNA was significantly increased in other three groups (P< 0.05). The expression level of PPARγ mRNA was the highest in ROS group. The expression level of PPARγ mRNA was re⁃ duced in GW group compared with that of I/R group and ROS group (P < 0.05). Conclusion Over expression of PPARγ may lead to the occurrence of malignant arrhythmia in myocardial ischemia/reperfusion rats.

Key words: PPAR-γ, Myocardial Ischemia/Reperfusion, Malignant Arrhythmia, Rosiglitazone