天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (7): 773-777.doi: 10.11958/j.issn.0253-9896.2015.07.020

• 临床研究 • 上一篇    下一篇

桂皮醛联合伏立康唑对临床分离烟曲霉菌的体外作用研究

罗劲，陈一强，孔晋亮，黄宏，侯长春，李冰，董必英   

  1. 广西医科大学第一附属医院呼吸疾病研究所
  • 收稿日期:2015-02-04 修回日期:2015-03-29 出版日期:2015-07-15 发布日期:2015-07-15
  • 通讯作者: 孔晋亮△ E-mail:chenyq2013@sohu.com
  • 基金资助:
    国家自然科学基金

In vitro efficacy of the combination of Cinnamaldehyde and Voriconazole on Aspergillusfumigatus isolates

LUO Jin, CHEN Yiqiang, KONG Jinliang△, HUANG Hong, HOU Changchun, LI Bing, DONG Biying   

  1. Institute of Respiratory Disease, First Affiliated Hospital of Guangxi Medical University, Nanning

  • Received:2015-02-04 Revised:2015-03-29 Published:2015-07-15 Online:2015-07-15
  • Contact: Jinliang△ E-mail:chenyq2013@sohu.com

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摘要： 摘要： 目的 评价桂皮醛与伏立康唑（VRC）联合应用对临床分离烟曲霉菌的体外抗菌活性。 方法 参照美国临床和实验室标准协会（CLSI）推荐的微量液基稀释法及棋盘法， 分别测定桂皮醛、VRC 单独使用及联合使用对 42 株临床分离烟曲霉菌的最小抑菌浓度（MIC）， 比较 2 药单用与联用的 MIC50、MIC90MIC 几何均数（MICG）及 MIC 分布； 绘制浓度-累积抑菌百分率曲线并计算分级抑菌指数（FICI）， 判断药物联合作用的类型。 结果 桂皮醛与 VRC联用后， 2 药的 MIC50、MIC90及 MICG 均较各自单用时明显降低（P＜ 0.01）， MIC 峰值分布及浓度-累积抑菌百分率曲线较单用时左移； 2 药联用的 FICI 值为 0.187 5~1.5， 其中 16 株（38.10%）体现为协同作用， 19 株（45.23%）为相加作用， 7 株（16.67%）为无关作用， 未出现拮抗作用。 受试菌株对 VRC 的敏感性越低， 2 药联用的 FICI 均数越小。 结论 桂皮醛与 VRC 联用对临床分离烟曲霉菌的体外联合抗菌效应主要表现为协同和相加作用， 且该药物组合对VRC 敏感性较低的烟曲霉菌表现出较强的协同抗菌作用。

关键词:  药物疗法, 联合, 体外研究, 桂皮醛, 伏立康唑, 烟曲霉菌

Abstract: Abstract： Objective To evaluate the in vitro antifungal activity of Cinnamaldehyde in combination with Voriconazole
(VRC) against clinically isolated Aspergillus fumigatus strains. Methods According to the Clinical and Laboratory Stan⁃
dards Institute (CLSI) M38-A2 document， the minimal inhibitory concentrations (MIC) of Cinnamaldehyde and VRC alone or
in combination against 42 clinical Aspergillus fumigatus isolates were determined by both microdillution method and check⁃
board method respectively. The MIC50, MIC90, MICG and MICs distribution were compared between single drug and both in
combination. The concentration-accumulative curve was drawn and fractional inhibitory concentration index (FICI) was cal⁃
culated to evaluate the interaction between two test agents. Results The values of MIC50, MIC90 and MICG were significant⁃
ly decreased (P＜ 0.001) when combination of the two drugs than those of their single use, with their MIC distribution concen⁃
tration-accumulative curves shifted to the left. The value of FICI of Cinnamaldehye-VRC combination ranged from 0.187 5
to 1.5. Sixteen strains (38.10%) of them showed the synergistic effect, 19 strains (45.23%) showed additive effect, and 7
strains (16.67%) showed an unrelated effect, and no antagonist effect on tested Aspergillus fumigatus strains in vitro. Conclu⁃
sion Cinnamaldehye in combination with VRC mainly shows a combined synergic and additive inhibitory effect on Asper⁃
gillus fumigatus isolates, and this combination appears to be more active against the test strains, which are less susceptible to
voriconazole.

Key words: drug therapy, combination, in vitro, Cinnamaldehyde, Voriconazole, Aspergillus fumigatus