天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (11): 1145-1150.doi: 10.11958/20180999

• 细胞与分子生物学 •    下一篇

基于公共数据库分析FABP5在肾透明细胞癌中表达及预后的意义

高鑫， 张淑芳△， 黄邓高， 曹卉   

  1. 中南大学湘雅医学院附属海口医院中心实验室 （邮编570208）
  • 收稿日期:2018-06-27 修回日期:2018-08-12 出版日期:2018-11-15 发布日期:2018-11-20
  • 通讯作者: 高鑫 E-mail:gaoxin_0612@csu.edu.cn
  • 基金资助:
    基于高通量测序技术的膀胱癌转录组分子标记的初步筛选;DDX46调节MDM2-p53反馈环促进膀胱癌增殖转移的机制研究;DDX46基因调控Wnt/β-Catenin通路影响膀胱癌增殖功能及其机制研究

Expression of FABP5 and its prognostic role in renal clear cell carcinoma: An analysis based on the data-mining of public database

GAO Xin, ZHANG Shu-fang△, HUANG Deng-gao, CAO Hui   

  1. Central Laboratory, Haikou People's Hospital, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou 570208, China
  • Received:2018-06-27 Revised:2018-08-12 Published:2018-11-15 Online:2018-11-20
  • Contact: xin gao E-mail:gaoxin_0612@csu.edu.cn

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摘要： 摘要： 目的 探讨脂肪酸结合蛋白5 （FABP5） 基因在肾透明细胞癌中的表达及预后意义。方法 提取Oncomine 和GEO数据库中有关FABP5的信息并进行表达情况分析， 通过DAVID数据库对差异基因进行功能注释， 采用 GEPIA进行预后分析。结果 Oncomine数据库中共找到445项FABP5在不同肿瘤类型中的研究数据集， 表达差异有统计学意义的结果中表达增高的有32项 （肾癌5项）， 表达降低的有24项 （肾癌0项）。肾癌中有4项涉及肾透明细胞癌和正常组织中FABP5的表达研究数据集。综合比较这4项数据集发现， FABP5在肾透明细胞癌中的表达量高于正常组织 （P＜0.05）。GEO数据库中GSE66271芯片数据分析结果发现差异基因FABP5在肾透明细胞癌中的表达量显著高于正常组织 （P＜0.01）。DAVID功能分析发现这些差异基因主要集中于影响细胞的转运蛋白活性和蛋白质的消化和吸收代谢功能。使用GEPIA分析来自TCGA数据库和GTEx项目的516例患者预后发现FABP5高表达的患者总体死亡率较高， 低表达FABP5的患者预后较好 （P=0.001 1）。结论 FABP5在肾透明细胞癌组织中呈现高表达，并与肾透明细胞癌预后差相关， 其有望成为抗肾透明细胞癌药物的重要治疗靶点。

关键词: 腺癌, 透明细胞, 自动数据处理, 数据库管理系统, FABP5基因, 肾透明细胞癌, Oncomine数据库, GEO数据库, DAVID数据库, GEPIA, 数据挖掘

Abstract: Abstract: Objective To explore the expression of fatty acid-binding protein 5 (FABP5) gene and its prognostic significance in renal clear cell carcinoma. Methods Data of FABP5 were retrieved from Oncomine and GEO database, then the role of FABP5 in renal clear cell carcinoma was mined and analyzed. The DAVID database was used to analyze the function of differentially genes, and the prognosis analysis was analyzed by GEPIA. Results Totally, 445 different types of cancer studies about the expression of FABP5 were identified in the Oncomine database. The FABP5 expression was statistically significant in 63 of the studies, over-expressed in 32 (5 in renal carcinoma) and under-expressed in the other 24 (0 in renal carcinoma). There were 4 studies involved the expression of FABP5 in clear cell renal cell carcinoma and normal tissues. Compared with normal tissues, the expression of FABP5 was higher in renal clear cell carcinoma (P＜0.05). In addition, through further mining the data set GSE66271 of GEO database, the results showed that the expression of FABP5 was higher in renal clear cell carcinoma than that in normal tissue (P＜0.001). The results of GSE66271 microarray data analysis in GEO database showed that the expression of differential gene FABP5 was significantly higher in renal clear cell carcinoma than that of normal tissue (P＜0.01). DAVID functional analysis found that these differential genes mainly focus on the affectting transfer protein activity and protein digestion and absorption metabolic function. The overall mortality was higher in patients with over-expressed FABP5 (n=258), and the prognosis was better in patients with under-expressed FABP5 (n=258) analyzed by GEPIA using TCGA database and GTEx project (P=0.001 1). Conclusion FABP5 is highly expressed in renal clear cell carcinoma, and its expression is associated with poor prognosis of renal clear cell carcinoma, which might be an important therapeutic target of renal clear cell carcinoma.

Key words:  adenocarcinoma, clear cell, automatic data processing, database management systems, FABP5 gene, renal clear cell carcinoma, oncomine database,  GEO database, DAVID database, GEPIA, data mining