天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (5): 479-483.doi: 10.11958/20190116

• 实验研究 • 上一篇    下一篇

机械加载减轻骨关节炎小鼠的病理进展

于咏 1，李心乐 1，2，刘大全 1，2，张平 1，2△   

  1. 基金项目：国家自然科学基金资助项目（81572100、81772405、81601863）；天津滨海新区卫生计生委科技资助项目（2016BWKL002、 2016BWKZ005） 作者单位：1天津医科大学基础医学院人体解剖与组织胚胎学系（邮编300070）；2北京协和医学院泰达国际心血管病医院再生医学中心 作者简介：于咏（1994），男，硕士在读，主要从事机械加载与骨关节炎疾病研究 △通讯作者 E-mail: pizhang2008@163.com
  • 收稿日期:2019-01-16 修回日期:2019-03-25 出版日期:2019-05-15 发布日期:2019-05-15
  • 通讯作者: 张平 E-mail:pizhang2008@163.com
  • 基金资助:
    机械加载通过调控骨重建和内质网应激治疗骨关节炎的机制研究;Salubrinal通过调节内质网应激治疗骨质疏松的机制研究;脊柱机械加载调控血管生成和骨重建治疗骨质疏松的机制研究

Mechanical loading reduces pathological progress in mice with osteoarthritis

YU Yong1, LI Xin-le1, 2, LIU Da-quan1, 2, ZHANG Ping1, 2△   

  1. 1 Department of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; 2 TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College △Corresponding Author E-mail: pizhang2008@163.com
  • Received:2019-01-16 Revised:2019-03-25 Published:2019-05-15 Online:2019-05-15

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摘要： 摘要：目的 探讨机械加载对骨关节炎（OA）模型小鼠早、晚期OA的治疗作用。方法 选用8周龄的C57BL/6 雌性小鼠36只，按随机数字表法分为正常对照组（Control组）、OA组和OA机械加载治疗组（OAL组），每组又分为2 周和8周亚组（n=6）。使用HE和番红O/快绿染色观察机械加载治疗对早、晚期OA小鼠软骨、滑膜和骨赘的治疗作 用。结果 与Control组相比，OA小鼠透明软骨（HC）厚度、全部关节软骨（TAC）厚度均变薄，国际骨关节炎研究学会 （OARSI）评分、滑膜评分、骨赘评分均升高（均P＜0.05）。机械加载治疗后，OAL组HC、TAC厚度均增加，OARSI评 分、滑膜评分、骨赘评分均下降（均P＜0.05）。相对数据表明，与早期OA小鼠相比，晚期小鼠的病理变化更严重，并 且机械加载对晚期OA的治疗效果更好。相关性分析表明，OA早期软骨的退化可能与滑膜炎症有关，而晚期可能与 骨赘形成有关。结论 机械加载可以改善OA小鼠的病理改变，可以作为一种新型的临床治疗手段。

关键词: 骨关节炎, 软骨, 关节, 滑膜炎, 骨赘, 机械加载

Abstract: Abstract: Objective To investigate the therapeutic effects of mechanical loading on early and late osteoarthritis (OA) of model mice. Methods Thirty-six 8-week-old female C57BL/6 mice, weighing about 20 g, were divided into the normal control (Control) group, OA group, and OA + loading (OAL) group by a random number table method. Each group was subdivided into 2-week and 8-week sub-groups (n=6). H&E and Safranin O/fast green staining were used to evaluate the therapeutic effects of mechanical loading on cartilage, synovium and osteophyte in early and late OA mice. Results Compared with the Control group, the thickness of hyaline cartilage (HC) and the thickness of all articular cartilage (TAC) were thinner in OA mice, and the OARSI score, synovial score and osteophyte score were significantly increased (all P＜ 0.05). After mechanical loading, the thickness of HC and TAC were increased in OAL group, and OARSI score, synovial score and osteophyte score were decreased (all P＜0.05). The relative data suggested that pathological changes were more severe in mice with late OA compared with mice of early OA, and the therapeutic effect of mechanical loading was better for the late OA. Correlation analysis showed that the early cartilage degradation of OA may be related to the synovial inflammation, and the late stage of OA may be related to the osteophyte formation. Conclusion Mechanical loading can improve the pathological changes of osteoarthritis mice, indicating that mechanical loading can be used as a new type of clinical treatment for osteoarthritis

Key words: osteoarthritis, cartilage, articular, synovitis, osteophyte, mechanical loading