Abstract: Objective　To explore the effect of stabilization splint combined with fixed orthodontic treatment on temporomandibular joint in class Ⅱ division 2 malocclusion adolescent female patients with temporomandibular disorders (TMD). Methods　Fifteen class Ⅱ division 2 malocclusion female patients with TMD were selected as the experimental group, and 15 class Ⅰ malocclusion female patients were used as the control group. The experimental group was treated with stabilization splint and fixed orthodontic appliance. At each stage of the treatment, cone beam CT was taken to measure the joint structure and the position of condyle. Results　The anterior space (Sa), superior space (Sp) and internal space (Sm) of temporomandibular joint (TMJ) were larger in the experimental group than those of the control group, while the posterior space (Sp), the inter-external diameter of the condyle (M-L) and the height of the condyle (Hc) were smaller in the experimental group than those of the control group (P＜0.05). The results of TMJ measurement in each stage of treatment in the experimental group were as follows: compared with T0 stage, Sa decreased, Su, Sp, the antero-posterior diameter of the condyle (A-P) and M-L increased in T1 stage (P＜0.05), while in T2 stage Sa decreased, Sp, A-P, M-L, Hc and the depth of the articular fossa (Hf) increased (P＜0.05). In addition, compared with T1 stage, Su decreased and Hf increased in T2 stage (P＜0.05). In the experimental group, the proportion of condylar posterior position was significantly lower at T1 stage and T2 stage than that at T0 stage (P＜0.05). Conclusion　The position and structure of TMJ in class Ⅱ division 2 malocclusion adolescent female patients with TMD are different from those of the normal people. The stabilization splint combined with orthodontic treatment is beneficial to those patients. There are obvious changes of joint space and condylar structure after treatment.