天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (1): 7-11.doi: 10.11958/20170840

• 细胞与分子生物学 • 上一篇    下一篇

补肾养骨汤诱导多发性骨髓瘤细胞株 KM3 的 凋亡及机制探讨

方梓青 1，黄幸儒 1，陈旭 1，李志远 2，李振杰 3，张建国 1，方坚 4△   

  1. 1 广州中医药大学第三临床医学院（邮编 510000）；2 广州市正骨医院；3 广州市荔湾区骨伤科医院；4 广州中医药大学第三附 属医院
  • 收稿日期:2017-07-26 修回日期:2017-11-03 出版日期:2018-01-15 发布日期:2018-01-16
  • 通讯作者: 方坚 E-mail:3369040042@qq.com
  • 作者简介:方梓青（1991），男，硕士研究生在读，主要从事中医药治疗骨伤科疾病的研究
  • 基金资助:
    广东省科技计划项目

Inductive effect of BuShenYangGuTang on apoptosis of multiple myeloma cell line KM3 and its mechanism

FANG Zi-qing1, HUANG Xing-ru1, CHEN Xu1, LI Zhi-yuan2, LI Zhen-jie3, ZHANG Jian-guo1, FANG Jian4△   

  1. 1 The Third Clinical Medical College of Guangdong University of Traditional Chinese Medicine, Guangzhou 510000, China; 2 Guangzhou Orthopedic Hospital; 3 Guangzhou City Liwan District Orthopedic Hospital; 4 The Third Affiliated Hospital of Guangzhou University of Chinese Medicine
  • Received:2017-07-26 Revised:2017-11-03 Published:2018-01-15 Online:2018-01-16

PDF (PC)

3644

摘要： 目的 探讨补肾养骨汤对人多发性骨髓瘤细胞株 KM3 细胞增殖、凋亡的影响及其机制。方法 取对数生 长期 KM3 细胞，分别加入 5%、10%、20% 的补肾养骨汤含药血清培养，培养 12、24、48 和 72 h 后，采用 Cell Counting Kit-8（CCK-8）法检测补肾养骨汤对 KM3 细胞增殖的影响；KM3 细胞经过不同浓度含药血清培养 72 h 后，采用流式 细胞术检测补肾养骨汤对 KM3 细胞周期和凋亡作用；RT-qPCR 和 Western blot 检测 B 淋巴细胞瘤-2 基因（Bcl-2）、 Bcl-2 相关蛋白（Bax）和核转录因子（NF）-κB 的表达水平。结果 补肾养骨汤抑制 KM3 细胞株的增殖，诱导 KM3 细胞株呈剂量依赖性凋亡，使 KM3 细胞周期停滞在 G0/G1 期，导致 KM3 细胞株 Bcl-2、NF-κB 表达下调，Bax 表达 上调。结论 补肾养骨汤抑制 KM3 细胞的增殖，影响细胞周期，诱导其凋亡，此作用机制可能与 Bcl-2、Bax 和 NF- κB 表达异常有关。

关键词: 多发性骨髓瘤, 细胞凋亡, 细胞增殖, 细胞周期, 补肾养骨汤, KM3 细胞

Abstract: Objective To study the effects and mechanisms of BuShenYangGuTang on proliferation and apoptosis of multiple myeloma cell line KM3. Methods The inhibitory effect of BuShenYangGuTang on cell proliferation was assessed by CCK-8. BuShenYangGuTang induced KM3 cell cycle arrest was analyzed by flow cytometry after propidium iodide staining. Flow cytometry was also used to analyze the cell apoptosis after Annexin V-FITC staining. The expressions of Bcl- 2, Bax and NF- κB were determined by RT-qPCR and Western blot assay. Results BuShenYangGuTang inhibited the KM3 cell proliferation in a dose-dependent manner. The expression levels of Bcl-2 and NF - κB were decreased, the expression level of Bax was increased, and the cell cycle was arrested in G0 / G1 phase after treatment with BuShenYangGuTang. Conclusion BuShenYangGuTang could inhibit the proliferation, arrest cell cycle and induce the apoptosis in KM3 cells, which may be related to the abnormal expressions of Bcl-2, Bax and NF-κB.

Key words: multiple myeloma, apoptosis, cell proliferation, cell cycle, BuShenYangGuTang, KM3 cells