天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (2): 166-170.doi: 10.11958/20171224

• 临床研究 • 上一篇    下一篇

SIRT1的表达在食管鳞状细胞癌侵袭转移中的临床意义

孔德华1 ， 曾薇1 ， 朱金峰2 ， 单莉1△   

  1. 1 新疆医科大学附属肿瘤医院肺内一科 （邮编830000）， 2 胃肠外科
  • 收稿日期:2017-11-07 修回日期:2017-12-11 出版日期:2018-02-15 发布日期:2018-02-15
  • 通讯作者: 单莉 E-mail:xjshanli@163.com
  • 基金资助:
    miR-34a/SIRT1 通过 PI3K/Akt 通路影响食管鳞癌侵 袭转移的机制研究

Clinical significance of SIRT1 expression in invasion and metastasis of esophageal squamous cell carcinoma

KONG De-hua1 , ZENG Wei 1 , ZHU Jin-feng2 , SHAN Li 1△   

  1. 1 Department of Oncology, 2 Department of Gastrointestinal Surgery, the Affiliated Tumor Hospital, Xinjiang Medical University, Xinjiang 830000, China
  • Received:2017-11-07 Revised:2017-12-11 Published:2018-02-15 Online:2018-02-15

PDF (PC)

4245

摘要： 摘要： 目的 观察食管鳞状细胞癌患者癌组织中沉默信息调节因子 1 （SIRT1） 的表达水平， 探讨其与食管鳞癌侵袭转移相关性及其对生存预后的影响。方法 回顾性分析 2009 年 1 月—12 月于新疆医科大学附属肿瘤医院行食管癌根治术的 104 例食管癌患者的临床及病理资料。采用免疫组化 （EnVision） 法检测癌组织与癌旁组织中 SIRT1 的表达水平， 比较不同临床病理特征 SIRT1 的表达情况， 并对食管癌患者的预后影响因素进行分析。结果 SIRT1 蛋白在食管鳞癌组织中的阳性表达率高于癌旁组织 ［61.54% （64/104）vs. 29.81% （31/104）， χ2 =21.100， P＜0.05］。食管鳞癌中 SIRT1 的阳性表达与脉管浸润 （VI）、 浸润深度 （pT）、 淋巴结转移 （pN）、 肿瘤临床分期 （pTNM） 密切相关 （均 P＜0.05）。SIRT1 阳性表达者的总生存时间 （OS） 低于阴性表达者 （Log-rank χ2 =10.065， P＜0.05）。单因素生存分析显示， SIRT1 阳性表达、 淋巴结转移、 浸润深度较深、 临床分期偏晚、 脉管浸润均提示不良预后 （均 P＜0.05）。Cox 多因素回归分析显示， SIRT1 阳性表达、 淋巴结转移、 浸润深度、 临床分期是食管鳞癌患者生存预后的独立影响因素， 且肿瘤浸润深、 淋巴结转移阳性、 临床分期晚、 SIRT1 高表达的患者预后更差。结论 SIRT1 在食管鳞癌中高表达， 其与食管鳞癌侵袭转移存在密切关系， 并对患者生存预后产生影响。

关键词: 关键词： 肿瘤侵润, 肿瘤转移, 预后, 食管鳞癌, SIRT1, 侵袭转移

Abstract: Abstract: Objective To observe the expression of silent information regulator 1 (SIRT1) in esophageal squamous cell carcinoma, and its relationship with invasion and metastasis of esophageal squamous cell carcinoma and its effect on survival and prognosis. Methods The clinical and pathological data of 104 patients with esophageal cancer who underwent radical esophagectomy at the Affiliated Tumor Hospital of Xinjiang Medical University from January to December 2009 were retrospectively reviewed. Immunohistochemical method (EnVision method) was used to detect the expression of SIRT1 in cancer tissues and adjacent tissues. The clinicopathological features of different SIRT1 expressions were compared. The factors affecting the prognosis of the patients with esophageal cancer were analyzed. Results The positive expression rate of SIRT1 protein was significantly higher in esophageal squamous cell carcinoma tissues than that in adjacent tissues (61.54% vs. 29.81%, χ2 =21.100, P＜0.05). The expression of SIRT1 in esophageal squamous cell carcinoma was significantly correlated with vascular infiltration (VI), infiltration depth (pT), lymph node metastasis (pN) and clinical stage (pTNM) (P＜ 0.05). Univariate survival analysis showed that the overall survival time (OS) was significantly lower in patients with SIRT1 positive expression than that of patients with negative expression (Log-rank χ2 =10.065， P＜0.05). SIRT1 positive expression, lymph node metastasis, deep tumor infiltration, late clinical stage and vascular infiltration showed poor prognosis (P＜0.05). Cox multivariate regression analysis showed that SIRT1 positive expression, lymph node metastasis, depth of invasion and clinical stage were independent prognostic factors for esophageal squamous cell carcinoma. The prognosis of patients with deep tumor invasion, lymph node metastasis, late clinical stage and high expression of SIRT1 was worse. Conclusion SIRT1 is highly expressed in esophageal squamous cell carcinoma, and which is closely related to the invasion and metastasis of esophageal squamous cell carcinoma and has an effect on the prognosis of patients.

Key words: Key words: neoplasm invasiveness, neoplasm metastasis, prognosis, esophageal squamous cell carcinoma, SIRT1, invasion and transfer