天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (11): 1166-1170.doi: 10.11958/20180910

• 诊断技术 • 上一篇    下一篇

肾康注射液抑制体外培养小鼠腹膜间皮细胞中TNF-α、 TGF-β1、 VEGF、 CTGF的释放

王荔1 ， 夏天1△， 张洪震2 ， 李荣1   

  1. 1天津医科大学第二医院肾内科 （邮编300211）， 2检验科
  • 收稿日期:2018-06-09 修回日期:2018-08-21 出版日期:2018-11-15 发布日期:2018-11-20
  • 通讯作者: 夏天 E-mail:xia_tian0204@163.com
  • 基金资助:
    天津市卫计委中医中西医结合科研课题

Shenkang injection can inhibit the release of TNF-α, TGF-β1, VEGF and CTGF in the cultured mouse peritoneal mesothelial cells in vitro

WANG Li 1 , XIA Tian1△, ZHANG Hong-zhen2 , LI Rong1   

  1. 1 Department of Nephrology, 2 Department of Clinical Laboratory, the Second Hospital of Tianjin Medical University, Tianjin 300211, China
  • Received:2018-06-09 Revised:2018-08-21 Published:2018-11-15 Online:2018-11-20

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摘要： 摘要： 目的 探讨肾康注射液对体外培养的小鼠腹膜间皮细胞 （PMCs） 的肿瘤坏死因子 （TNF） -α、 转化生长因子（TGF） -β1、 血管内皮生长因子 （VEGF）、 结缔组织生长因子 （CTGF） 等细胞因子水平的影响。方法 小鼠PMCs经传代培养及鉴定后， 分为空白对照组 （A组， DMEM）； 阳性对照组 （B组， DMEM+ 140 mmol/L葡萄糖）； 低 （C组）、 中 （D 组）、 高 （E组） 剂量肾康干预组 （B组培养液+含体积比为1%、 2%、 4%肾康注射液）。培养24 h后分别收集细胞和培养上清液， 采用酶联免疫吸附试验检测培养上清液中TNF-α、 TGF-β1、 VEGF、 CTGF水平， Real-time RT-PCR检测 PMCs中TNF-α、 TGF-β1、 VEGF、 CTGF的mRNA转录水平。结果 与A组比较， B组TNF-α、 TGF-β1、 VEGF、 CTGF 浓度及mRNA表达水平均明显上升 （P＜0.05）； 与B组比较， 加入肾康注射液后， C、 D、 E组培养上清液中由高糖诱导的TNF-α、 TGF-β1、 VEGF、 CTGF浓度及相应mRNA水平均出现不同程度下降 （P＜0.05）， 并呈现一定的剂量依赖性。结论 一定浓度的肾康注射液可抑制高糖诱导的小鼠PMCs中TNF-α、 TGF-β1、 VEGF、 CTGF的表达和转录。

关键词: 肾疾病, 腹膜后纤维化, 细胞因子类, 肾康注射液, 小鼠腹膜间皮细胞

Abstract: Abstract: Objective To investigate the effect of Shenkang injection on levels of cytokines, such as tumor necrosis factor-alpha (TNF - α), transforming growth factor-beta 1 (TGF - β1), vascular endothelial growth factor (VEGF) and connective tissue growth factor (CTGF) of mouse peritoneal mesothelium cells (MPMCs) cultured in vitro. Methods MPMCs were cultured and identified, and divided into blank control group (group A, DMEM), positive control group (group B, DMEM+140 mmol /L glucose), low (group C), medium (group D) and high (group E) doses of Shenkang injection groups (culture medium of B group + volume ratio 1%, 2%, 4% Shenkang injection). MPMCs and culture supernatamt were collected respectively after being cultured for 24 hours. The levels of TNF-α, TGF-β1, VEGF and CTGF in the supernatant were detected by enzyme-linked immunosorbent assay. The levels of mRNA transcription of these four cytokines were detected by real-time RT-PCR. Results Compared with group A, TNF-α, TGF-β1, VEGF and CTGF levels in culture supernatant and mRNA levels in cultured MPMCs were significantly increased in group B (P＜0.05). Compared with group B, after adding Shenkang injection to cell culture medium, all of TNF-α, TGF-β1, VEGF and CTGF induced by high- concentration glucose and mRNA levels in cultured MPMCs were decreased with a dose-dependent way. Conclusion A certain concentration of Shenkang injection can inhibit the expression and transcription of TNF- α, TGF-β1, VEGF and CTGF induced by high-concentration glucose in MPMCs.

Key words: kidney diseases, retroperitoneal fibrosis, cytokines, Shenkang injection, mice peritoneal mesothelial cells