天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (1): 10-13.doi: 10.11958/20181297

• 细胞与分子生物学 • 上一篇    下一篇

CHOP疗法对T细胞淋巴瘤Hut 78和HH细胞环状 RNA circ_001569及其下游蛋白表达的影响

康庆伟，阎姝△   

  1. 作者单位：天津市南开医院药学部（邮编300100） 作者简介：康庆伟（1970），女，本科，副主任药师，主要从事临床药理学方面研究 △通讯作者 E-mail: 13389989966@126.com
  • 收稿日期:2018-08-28 修回日期:2018-12-10 出版日期:2019-01-15 发布日期:2019-01-15
  • 通讯作者: 阎姝 E-mail:13389989966@126.com

Effects of CHOP therapy on the expression of cyclic RNA circ_001569 and its downstream protein in T-cell lymphoma Hut 78 and HH cell lines

KANG Qing-Wei, YAN Shu△   

  1. Department of Pharmacy, Tianjin Nankai Hospital, Tianjin 300100, China
  • Received:2018-08-28 Revised:2018-12-10 Published:2019-01-15 Online:2019-01-15

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摘要： 目的 探索 CHOP 疗法对 T 细胞淋巴瘤细胞中环状 RNA circ_001569 及其下游功能蛋白的影响。方法 分别采用CHOP疗法中的4种化疗药物［环磷酰胺（1 μmol/L）、羟基柔红霉素（0.2 μmol/L）、长春新碱（1 μmol/L）、泼尼 松（1 μmol/L）］处理T细胞淋巴瘤Hut78和HH细胞，以常规培养细胞作为对照。分组处理24 h后检测各组细胞增殖 情况，采用特异性逆转录环状 RNA 和茎环法逆转录 miRNA，实时定量 PCR 检测各组 circRNA_001569、miR-145 和 BAG4 mRNA的表达水平，Western Blot检测BAG4蛋白表达。结果 4种药物均可显著抑制Hut78细胞和HH细胞增 殖（P＜0.05）。经过羟基柔红霉素、长春新碱处理后，Hut78和HH细胞circRNA_001569的表达水平降低，其下游分子 miR-145 表达上调，BAG4 mRNA 和蛋白表达下调。结论 CHOP 疗法治疗 T 细胞淋巴瘤的过程中，环状 RNA circRNA_001569可能起到了重要的调控作用。

关键词: 淋巴瘤, T细胞, 抗肿瘤联合化疗方案, 环状RNA, circRNA_001569, BAG家族分子伴侣蛋白调节因子4

Abstract: Objective To explore the effect of CHOP therapy on the cyclic RNA and its downstream function proteins in T cell lymphoma cells. Methods Hut78 and HH cells were treated with cyclophosphamide (1 μmol / L), hydroxydaunorubicin (0.2 μmol/L), oncovin (1 μmol/L) or prednisone (1 μmol/L) for 24 h. Conventional cultured cells were used as control. The expression levels of circRNA_001569, miR-145 and BAG4 mRNA were detected by the qPCR method, and the expression of BAG4 was detected by qPCR and Western blot assay. Results Hydroxydaunorubicin and oncovin could decrease the expression levels of circRNA_001569, promote miR-145, and down-regulate the mRNA and protein expression level of BAG4 in Hut78 and HH cell lines. Conclusion CircRNA_001569 may play an important role in the course of CHOP therapy for T cell lymphoma.

Key words: lymphoma, T-cell, antineoplastic combined chemotherapy protocols, circRNA, circRNA_001569, BAG family molecular chaperone regulator 4