天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (9): 813-817.doi: 10.11958/20193099

• 细胞与分子生物学 • 上一篇    下一篇

TRAP1对结直肠癌细胞增殖和凋亡的影响

李品玉1，盛文杰1，张嫄怡1，杨坚1，郑培丽1，张菲菲2   

  1. 1肇庆医学高等专科学校病理学与病理学教研室（邮编526000）；2南方医科大学南方医院整形外科
  • 收稿日期:2019-10-17 修回日期:2020-07-09 出版日期:2020-09-15 发布日期:2020-09-15
  • 作者简介:李品玉（1985），男，硕士，讲师，主要从事消化道肿瘤病理学研究和病理学教学
  • 基金资助:
    广东省普通高校青年创新人才类项目（2018GkQNCX120）

Effects of TRAP1 on proliferation and apoptosis of colorectal cancer cells

LI Pin-yu1, SHENG Wen-jie1, ZHANG Yuan-yi1, YANG Jian1, ZHENG Pei-li1, ZHANG Fei-fei2   

  1. 1 Department of Pathology and Pathophysiology, Zhaoqing Medical College, Zhaoqing 526000, China; 2 Department of Plastic and Aesthetic Surgery, Nanfang Hospital, Southern Medical University
  • Received:2019-10-17 Revised:2020-07-09 Published:2020-09-15 Online:2020-09-15

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摘要： 摘要：目的　探讨肿瘤坏死因子受体相关蛋白1（TRAP1）在结直肠癌中的表达情况及对结直肠癌细胞增殖和凋亡的影响。方法　通过Oncomine数据库分析TRAP1在结直肠癌组织和正常肠黏膜组织中的表达，Western blot检测TRAP1在结直肠癌组织及其配对癌旁黏膜组织、LOVO、HT29、HCT116、RKO、SW480、NCM460细胞中的表达及干扰TRAP1对细胞周期相关蛋白、凋亡相关蛋白及PI3K/AKT通路的影响，CCK-8检测TRAP1对结直肠癌细胞增殖的影响，平板克隆实验检测TRAP1对结直肠癌细胞克隆形成能力的影响，流式细胞术检测TRAP1对细胞周期及凋亡的影响。结果　生物信息学分析显示TRAP1在结直肠癌中的表达高于正常肠黏膜组织，Western blot结果显示TRAP1在结直肠癌组织和细胞中蛋白表达水平均高于正常组织和细胞（P＜0.05），干扰TRAP1的表达可以显著抑制SW480细胞的增殖及克隆形成能力（P＜0.05），并且将细胞阻滞在G0/G1期，增加细胞凋亡（P＜0.01），细胞周期相关蛋白CyclinD1表达降低，凋亡相关蛋白Cleaved-Casp3表达增加，p-PI3K和p-AKT表达降低（P＜0.05）。结论　TRAP1在结直肠癌中高表达，干扰TRAP1的表达可以通过抑制PI3K/AKT通路的激活，从而降低结直肠癌细胞的增殖能力，促进细胞凋亡。

关键词: 结直肠肿瘤；细胞系, 肿瘤；RNA干扰；细胞增殖；细胞凋亡；肿瘤坏死因子受体相关蛋白1

Abstract: Abstract: Objective　To investigate the expression of tumor necrosis factor receptor-associated protein 1 (TRAP1) in colorectal cancer and its effects on proliferation and apoptosis of colorectal cancer cells. Methods　The Oncomine database was used to analyze the expressions of TRAP1 in colorectal cancer tissues and normal intestinal mucosal tissues. Western blot assay was used to detect the expressions of TRAP1 in colorectal cancer tissues and their paired paracancerous mucosa tissues, LOVO, HT29, HCT116, RKO, SW480 and NCM460 cells. CCK-8 and plate cloning assay were used to detect the effects of TRAP1 on the proliferation of colorectal cancer cells. Flow cytometry was used to detect the effects of TRAP1 on cell cycle and apotosis. The effects of TRAP1 on cell cycle-associated protein, apoptosis-related protein and PI3K/AKT pathway markers were detected by Western blot assay. Results　Bioinformatics analysis showed that TRAP1 was highly expressed in colorectal cancer. Western blot results showed that the protein expression levels of TRAP1 were higher in colorectal cancer cells and tissues than those in normal tissues and cells (P＜0.05). Interfering TRAP1 expression with siRNA significantly inhibited cell proliferation and clonality (P＜0.05), and cells were arrested in G0/G1 phase, which increased apoptosis (P＜0.01). The expression of cell cycle-associated protein CyclinD1 was decreased, the expression of apoptosis-related protein Cleaved-Casp3 was increased and the expressions of p-PI3K and p-AKT were decreased (P＜0.05). Conclusion　TRAP1 is highly expressed in colorectal cancer. Interfering the expression of TRAP1 can inhibit the proliferation of colorectal cancer cells and promote apoptosis by inhibiting the activation of PI3K/AKT pathway.

Key words: colorectal neoplasms, cell line, tumor, RNA interference, cell proliferation, apoptosis, TRAP1

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