天津医药

天津医药 ›› 2021, Vol. 49 ›› Issue (9): 904-909.doi: 10.11958/20210599

• 细胞与分子生物学 • 上一篇    下一篇

长链非编码RNA BCYRN1对肺腺癌细胞生物学行为的影响

李世雄1，2，耿华2，徐美林2△   

  1. 1天津医科大学胸科临床学院病理科（邮编300222）；2天津市胸科医院病理科
  • 收稿日期:2021-03-12 修回日期:2021-06-27 出版日期:2021-09-15 发布日期:2021-09-18
  • 通讯作者: 徐美林 E-mail:meilinxu-gh@163.com
  • 基金资助:
    天津市卫生和计划生育委员会科技基金项目（16KG133）

Effects of long non-coding RNA BCYRN1 on the biological behavior of lung adenocarcinoma cells#br#

LI Shi-xiong1, 2, GENG Hua2, XU Mei-lin2△   

  1. 1 Department of Pathology, Clinical College of Chest, Tianjin Medical University, Tianjin 300222, China; 
    2 Department of Pathology, Tianjin Chest Hospital
  • Received:2021-03-12 Revised:2021-06-27 Published:2021-09-15 Online:2021-09-18
  • Contact: XUMEILIN E-mail:meilinxu-gh@163.com

PDF (PC)

2977

摘要： 摘要：目的　探究长链非编码RNA（lncRNA）脑胞质RNA1（BCYRN1）在肺腺癌细胞中的表达及对细胞周期、凋亡、侵袭和迁移能力的影响。分析BCYRN1表达与肺腺癌患者临床病理特征及预后的关系。方法　分别培养正常肺上皮细胞BEAS-2B和肺腺癌细胞A549、H1299，采用实时荧光定量聚合酶链反应（qPCR）检测BCYRN1在正常细胞和肺腺癌细胞中的表达水平；分别将BCYRN1敲低及对照质粒转染入H1299肺腺癌细胞，流式细胞仪分析细胞周期和凋亡的变化；通过划痕实验、Transwell实验检测细胞侵袭和迁移能力的变化。结合癌症基因组图谱（TCGA）数据库，分析BCYRN1表达与肺腺癌患者临床病理特征的相关性。利用癌症阵列的lncRNA（lnCAR）数据库中lncRNAs表达数据，采用Cox回归分析BCYRN1表达与患者预后的关系。结果　BCYRN1在肺腺癌细胞中的表达水平显著高于肺正常上皮细胞（P＜0.05）；流式细胞分析提示BCYRN1敲低组的细胞出现S期阻滞，细胞凋亡率增加；划痕实验和Transwell实验显示BCYRN1敲低组H1299细胞侵袭和迁移能力减弱。生物信息学分析显示BCYRN1的表达与患者的N分期和肿瘤分期相关（P＜0.05），BCYRN1高表达患者总生存期显著缩短。结论　LncRNA BCYRN1在肺腺癌细胞中高表达，下调BCYRN1可抑制肺腺癌细胞的侵袭和迁移能力，并使细胞周期发生阻滞。其高表达与肺腺癌患者N分期和肿瘤分期相关，BCYRN1高表达患者预后较差。

关键词: RNA, 长链非编码；肺肿瘤；腺癌；计算生物学；细胞增殖；细胞运动；脑胞质RNA1

Abstract: Abstract: Objective　To investigate the expression of long non-coding RNA (lncRNA) brain cytoplasmic RNA 1 (BCYRN1) in lung adenocarcinoma cell lines A549 and H1299 and its effects on cell cycle, apoptosis, invasion and migration, then to analyze the relationship between the expression of BCYRN1 and clinicopathological features and prognosis of patients with lung adenocarcinoma. Methods　Normal lung epithelial cells BEAS-2B and lung adenocarcinoma cells A549 and H1299 were cultured. Real-time quantitative polymerase chain reaction (qPCR) was used to detect the expression level of BCYRN1 in normal cells and lung adenocarcinoma cells. BCYRN1 down-regulation and control plasmid were transfected into H1299 lung adenocarcinoma cells, respectively. The cell cycle and apoptosis were analyzed by flow cytometry. Changes of cell invasion and migration ability were detected by scratch test and Transwell test. Combined with the Cancer Genome Atlas (TCGA) database, the correlation between BCYRN1 expression and clinicopathological characteristics of patients with lung adenocarcinoma was analyzed. Cox regression was used to analyze the relationship between BCYRN1 expression and patient prognosis by using the lncRNAs from cancer arrays (lnCAR) database. Results　The expression level of BCYRN1 was significantly higher in lung adenocarcinoma cells than that in normal lung epithelial cells (P＜0.05). Flow cytometry analysis indicated that S phase arrest and the proportion of cells in the apoptotic phase increased in the BCYRN1 knockdown cells. The scratch test and Transwell test showed that the invasion and migration of H1299 cells reduced in BCYRN1 knockdown group. Bioinformatics analysis showed that the expression of BCYRN1 was associated with N stage and tumor stage (P＜0.05), and the overall survival of patients with high BCYRN1 expression was significantly shortened. Conclusion BCYRN1 is highly expressed in lung adenocarcinoma cells. BCYRN1 knockdown inhibits the invasion and migration of lung adenocarcinoma and causes cell cycle arrest. The high expression of BCYRN1 is associated with N stage and tumor stage of lung adenocarcinoma. The prognosis of patients with high expression of BCYRN1 is poor.

Key words: long noncoding RNA, lung neoplasms, adenocarcinoma, computational biology, cell proliferation, cell movement, BCYRN1