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牛磺酸对脑创伤大鼠脑组织代谢的影响

常小丽   

  1. 天津市天和医院
  • 收稿日期:2011-07-28 修回日期:2011-11-17 出版日期:2012-05-15 发布日期:2012-05-15
  • 通讯作者: 常小丽

The Effect of Taurine on Train Metabolism in Rats Following with Severe Traumatic Brain Injury

CHANG Xiaoli   

  • Received:2011-07-28 Revised:2011-11-17 Published:2012-05-15 Online:2012-05-15
  • Contact: CHANG Xiaoli

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摘要： （1天津市天和医院，2天津市环湖医院，3天津医科大学） 摘要 目的 本课题主要研究牛磺酸对脑创伤后脑组织代谢的影响情况。方法 将30只雄性SD大鼠随机分为：假手术组（sham）、脑创伤组（TBI）及牛磺酸治疗组（TAU），每组10只。TBI组和TAU组用液压打击仪制作左侧脑创伤模型，TAU组于伤后立即从尾静脉给予牛磺酸治疗（200mg/kg）， sham组和TBI组给予等量的生理盐水。用微透析探针收集各组左侧脑组织的1-3h、22-24h、7d的脑组织间液，用CMA600全自动生化分析仪检测细胞间液的葡萄糖，乳酸，甘油，丙酮酸的含量并计算乳酸/丙酮酸的比值。结果（1）在创伤后7d内，三组左侧脑组织的葡萄糖含量无显著区别；TBI组的葡萄糖随时间的推移而显著升高。(2)TBI组的乳酸含量在3个时间点内均显著高于sham组，并随时间推移而显著下降；牛磺酸在24h后显著降低创伤后的乳酸含量，与sham组比无显著区别。（3）与sham组比较，脑创伤后伤侧脑组织的甘油无显著变化，而牛磺酸在7d时显著降低其含量。（4）TBI组的乳酸/丙酮酸比值在3个时间点内均显著高于sham组，并随时间而显著下降；牛磺酸显著降低创伤后的乳酸/丙酮酸比值，但均显著高于sham组。结论 脑创伤后脑组织间液中的乳酸、乳酸/丙酮酸比值显著升高，牛磺酸对调节脑创伤后的代谢紊乱有一定的积极作用。

关键词: 脑创伤, 能量代谢, 葡萄糖, 乳酸, 甘油, 丙酮酸

Abstract: Abstract Object The purpose of this research is to observe the effect of taurine on energy metabolism in rats following with traumatic brain injury. Methods 24 rats were randomly divided into sham group, traumatic brain injury group (TBI) and taurine treating group,8 rats in each group. The groups of TBI and taurine were subjected to severe traumatic injury of left brain by lateral fluid pressure device. Then taurine group were injected traurin（200mg/kg）and the other groups were given saline through caudal vein immediately. Intercellular fluid in each group(each group contain 3 time points,1-3h, 22-24h, and 7d) in left brain tissue were collected by using the method of microdialysis, glucose, lactate, glycerol, pyruvate in the samples were measured using CMA600 auto biochemistry analyzer, and calculate the ratio of lactate and pyruvate. Results (1) The level of glucose in left brain tissue had no statistic differences in the three groups after traumatic brain injury; But the level of glucose were increasing significantly in TBT group with time goes.(2)The level of lactate was all higher in TBI group at the three time than those in sham group，and the differences had statistic significance, whereas ,the level of lactate decreased in TBI group with time goes. Taurine could markedly decrease the content of lactate in 24h, which had no significant difference to sham group.(3) After traumatic brain injury, the level of glycerol in damaged hippocampus had no statistic differences to sham group, but it decreased markedly in taurine group.(4)The level of L/P were all higher in TBI group at the 3 time point than those in sham group, and decreased with time goes. The level of L/P in taurine group were all lower than that in TBI group at 3 time point, but it was still markedly higher than that in sham group. Conclution It can appear metabolism function disorder and taurine may make an active effect on adjusting metabolism disorder after traumatic brain injury.

Key words: traumatic brain injury, energy metabolism, glucose, lactate, glycerol, pyruvate