天津医药

• 实验研究 •    下一篇

氟伐他汀对心力衰竭大鼠心室重构及血管性血友病因子的影响

王士凯1,王海燕2,薛彦菊3,梁江久3   

  1. 1. 山东省宁阳县第一人民医院
    2. 山东大学附属千佛山医院超声诊疗科
    3. 山东大学附属千佛山医院保健科
  • 收稿日期:2012-10-18 修回日期:2013-01-11 出版日期:2013-08-15 发布日期:2013-08-15
  • 通讯作者: 王士凯

The Effect of Fluvastatin on Ventricular Remodeling and VWF in Heart Failure Rats

WANG shi kai1,WANG Haiyan 2,XUE Yanju 3,LIANG Jiangjiu 3   

  1. 1. Ningyang First People’ s Hospital, Shandong Taian 271400, China
    2. Department of Ultrasound, Qianfoshan Hospital Affiliated to Shandong University
    3. Health Division, Qianfoshan Hopital Affiliated to Shandong University
  • Received:2012-10-18 Revised:2013-01-11 Published:2013-08-15 Online:2013-08-15
  • Contact: WANG shi kai

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摘要： 【摘要】 目的 研究氟伐他汀对慢性心衰大鼠心室重构和心功能的保护作用， 并探讨其对血管性血友病因子（VWF）的影响。 方法 皮下注射异丙基肾上腺素（170 mg/kg）2次,建立心力衰竭大鼠模型， 将 18 只慢性心衰大鼠随机分为氟伐他汀组(20 mg ·kg-1·d-1)、安慰剂组和正常对照组， 安慰剂组和正常对照组给予等量生理盐水。 灌胃灌药 6周后， 超声心动图观察各组大鼠心脏结构和功能的变化， 测定心室质量分数， 酶联免疫吸附试验（ELISA）检测血浆VWF和 B 型脑钠肽（BNP)的含量， 逆转录多聚酶链反应（RT-PCR）检测心脏组织 VWF mRNA 的表达。 结果 与正常对照组相比较， 安慰剂组和氟伐他汀组左心室舒张末期内径（LVEDD）和左心室收缩末期内径（LVESD）均显著增加，左心室射血分数（LVEF）和左室射血分数缩短率（LVFS）均显著降低（P＜ 0.05）， 左心室湿质量/体质量(LVRW）及右室湿质量/体质量（RVRW）显著增加， 心脏组织 VWF mRNA 表达明显增强（P＜ 0.01）； 与安慰剂组相比， 氟伐他汀组LVEDD、LVRW 及 RVRW 均降低， 心脏组织 VWF mRNA 表达明显降低（P＜ 0.01）， LVEF 和 LVFS 均显著增加（P＜0.05）； VWF 与 BNP 呈高度正相关（r=0.996）。 结论 氟伐他汀能改善心室重构及心功能， 其作用机制可能与调节VWF的水平、改善内皮功能有关。

关键词: 心力衰竭, 心室重构, 利钠肽, 脑, 大鼠, Wistar, 氟伐他汀, 血管性血友病因子

Abstract: Abstract  Objective  To study the effect of fluvastatin on cardiac remodeling and cardiac function , and to investigate the effect of VWF. Method   CHF model was induced by soproterenol via subcutaneous injection and rats were randomly divided into Placebo group and fluvastatin group , Another normal 10 rats served as the control group. Fluvastatin group were treated with fluvastatin (20mg /kg/d), Placebo group and control group were treated with normal saline.After 6 weeks treatment,the structure and function of hearts in different groups were measured by echocardiography ;measured ventricular weight index, the serum levels of VWF and BNP were measured by ELISA; the levels of VWF mRNA in cardiac muscle were measured by RT-PCR. Results  Compared with the control group, LVEDD, LVESD, LVRW, RVRW and VWF mRNA were significant increased in the Placebo group and drug groups, while LVEF and LVFS were significant decreased;Compared with placebo group,LVEDD,LVESD,LVRW,RVRW and VWF mRNA were significant decreased in the drug groups, while LVEF and LVFS were significant increased in the drug groups;The level of VWF were positive correction with the BNP. Conclusion   Fluvastatin could improve the cardiac function and prevent the cardiac remodeling, the mechanisms may be by reducing the levels of VWF and improving endothelial function.

Key words: heart failure, ventricu1ar remodehng, natriuretic peptide, brain, rats, Wistar, Fluvastatin, 血管性血友病因子