天津医药

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儿童急性淋巴细胞白血病雌激素受体α与β的表达及临床意义

柯江维1,段荣2,钟小红2,刘志强2   

  1. 1. 江西省儿童医院
    2.
  • 收稿日期:2009-08-13 修回日期:2009-11-11 出版日期:2010-08-15 发布日期:2010-08-15
  • 通讯作者: 柯江维

Expression of estrogen receptor α and β in childhood acute lymphoblastic leukemia and clinical significance

  • Received:2009-08-13 Revised:2009-11-11 Published:2010-08-15 Online:2010-08-15

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摘要： [摘要] 目的：研究雌激素受体（ER）α与β在儿童急性淋巴细胞白血病（ALL）中的表达水平及与临床疗效的关系。方法：先采用白细胞免疫标记技术APAAP法对30例儿童急性淋巴细胞白血病（ALL）患儿进行免疫学分型，然后采用免疫组织化学SP法检测30例儿童急性淋巴细胞白血病患儿骨髓单个核细胞ERα与ERβ的表达；对照组为15例非恶性血液病患儿。结果：30例初诊组中ERα、ERβ阳性表达率分别为65.7%和70.7%，明显高于对照组（P<0.05）；ERα阳性表达者与阴性表达者之间缓解率无显著差异（P>0.05），ERβ阳性表达者缓解率明显高于ERβ阴性表达者，两者比较有显著差异（P<0.05）；在缓解组与未缓解组之间ERα阳性表达率无显著差异（P>0.05），在缓解组与未缓解组之间ERβ阳性表达率有显著差异（P<0.05）。在ALL-B和ALL-T中ERα、ERβ表达均无统计学意义。结论：儿童急性淋巴细胞白血病可能有一定的激素依赖性，ERβ在儿童急性淋巴细胞白血病发生、发展、预后评估上发挥重要角色。

关键词: 急性白血病, ERα, ERβ , 儿童, 免疫组织化学

Abstract: [Abstract] Objective To investigate the expression and relation of estrogen receptor(ER) α and β in childhood acute lymphoblastic leukemia(ALL). Methods: Fist using an immunocyto- chemical stain technique APAAP to immunophenotype thirty childhood with ALL in newly diagnosed group.The expression of ERα and ERβ in all patients’ mononuclear cells of bone marrow was detected by S-P immunohistochemical assay.The control group including 15 children with normal bone marrow. Results:The positive expression of ERα and ERβ is 56.7% and 76.7% respectively in the newly diagnosed ALL group, both were remarkably significant higher than that in the control group (P<0.05). The rate of the remission have no significant difference between the positive expression and negative expression of ERα(P>0.05). The rate of the remission of the positive expression of ERβ was remarkably significant higher than that of the negative expression of ERβ(P<0.05). The rate of the positive expression of ERα have no significant difference between the remission and non-remission group(P>0.05). The rate of the positive expression of ERβ in the remission group was remarkably significant higher than that in the non-remission group (P<0.05).The expression of ERα and ERβ were no difference respectively in ALL-B and ALL-T. Conclusions: Childhood ALL may be an incretion dependent tumor. ERβ may play an important role in the occurrence, development and prognosis of childhood ALL.

Key words: acute leukemia , ERα , ERβ, childhood , immunohistochemistry