天津医药

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缺血后处理对大鼠心肌细胞凋亡及Bcl-xl/s的影响

董福强1,田轶魁2,魏民新1,张 鑫1   

  1. 1. 天津医科大学总医院
    2.
  • 收稿日期:2011-08-02 修回日期:2011-08-18 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 魏民新

Apoptosis and expression of Bcl-xl/s in Postconditioning

  • Received:2011-08-02 Revised:2011-08-18 Published:2011-12-15 Online:2011-12-15
  • Contact: Minxin Wei

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摘要： 目的：从Bcl-xl/s基因和蛋白水平探讨缺血后处理抗细胞凋亡的机制。方法：18只健康wistar大鼠随机分为假手术（Sham）组、缺血再灌注损伤(I/R)组、缺血后处理（PostC）组，每组6只。采用原位缺口末端标记(TUNEL)检测大鼠心肌细胞凋亡指数；免疫组化法检测BcL-xl/s蛋白表达水平；荧光实时定量PCR法检测心肌组织Bcl-xl/s基因表达水平。结果：与I/R组相比，PostC组再灌注后心肌细胞凋亡指数明显降低，PostC组Bcl-xl 蛋白及mRNA表达水平明显上调，Bcl-xs 蛋白及mRNA表达水平明显下调。结论： PostC能显著减少缺血再灌注后心肌细胞凋亡，凋亡相关基因Bcl-xl、Bcl-xs mRNA及蛋白参与了由缺血再灌注诱导的心肌细胞凋亡。

关键词: 肌细胞, 心脏, 心肌再灌注损伤, 细胞凋亡, bcl-X蛋白质, 原位缺口末端标记, 免疫组织化学, 逆转录聚合酶链反应

Abstract: Objective：To study the anti-apoptosis and the expression of Bcl-xl/s in postconditioning. Methods: Healthy adult male wistar rats were divided into three groups. (1) Sham group, (2) I/R group, (3) PostC group，TUNEL staining was used to analysis myocardial apoptosis index; Immunohistochemisty was used to detect proteinum.The expression of Bcl-xl mRNA and Bcl-xs mRNA was determined by Real-time PCR. Results:Compared with I/R group,the cardiomyocyte apoptotic index decreased in PostC group, the expression of Bcl-xl protein increased, the expression of Bcl-xl mRNA increased,while the expression of Bcl-xs protein decreased, the expression of Bcl-xs mRNA decreased in PostC group. Conclusion: PostC can significantly decrease apoptosis, Bcl-xl、Bcl-xs mRNA and protein involved in the ischemia-reperfusion-induced myocardial apoptosis.

Key words: 逆转录聚合酶链反应