天津医药

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中波紫外线对角质形成细胞MMP-9及TIMP-1的影响

王晶晶1,车雅敏2,刘原君1,王璐1,柯吴坚2,3,刘全忠2,3   

  1. 1. 天津市和平区天津医科大学总医院
    2. 天津医科大学总医院皮肤科
    3.
  • 收稿日期:2011-10-20 修回日期:2011-12-04 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 车雅敏

Study On The UVB-Induced MMP-9And TIMP-1 mRNA expression on HaCaT cells

  • Received:2011-10-20 Revised:2011-12-04 Published:2012-07-15 Online:2012-07-15

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摘要： 【摘要】目的：研究中波紫外线(UVB)照射对HaCaT细胞中基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶抑制剂-1（TIMP-1）mRNA表达的影响。方法：培养HaCaT细胞，绘制细胞生长曲线，经不同剂量（0、30、60、90 mJ/cm2）UVB照射；用MTT方法测定UVB照射后细胞的增殖活性，反转录-聚合酶链反应（RT-PCR）方法测定UVB照射后HaCaT细胞中MMP-9 mRNA 和 TIMP-1 mRNA的表达。结果：随着照光剂量的增大，细胞的增殖活性逐渐降低，而细胞的增殖抑制率逐渐增加，不同照射剂量各组间OD值差异有统计学意义（P<0.05）；随着照光剂量的增大，MMP-9 mRNA的表达逐渐增加，各组之间比较差异均有统计学意义（P<0.05）；TIMP-1mRNA的表达逐渐降低，与0 mJ/cm2比较差异具有统计学意义（P<0.05）。结论：UVB照射可诱导HaCaT细胞损伤和细胞凋亡，MMP-9和TIMP-1可能与光老化的发生有一定关系。

关键词: 紫外线, 角蛋白质类, 光刺激, 皮肤衰老, 基质金属蛋白酶9, 金属蛋白酶1组织抑制剂

Abstract: [Abstract] Objective：To investigate the effect of UVB irradiation induced the matrix metalloproteinase (MMP-9) and Tissue inhibitor of Matrix Metalloproteinase (TIMP-1) mRNA expression on immortalized human keratinocyte cells (HaCaT cells)．Methods：Culture the HaCaT cells ,Draw cell growth curve, and the cells was treated with different doses (0、30、60、90 mJ/cm2)of UVB irradiation ,MTT method for determining cell proliferation.Semiquantitative-PCR (RT-PCR) was used to measure the expression levels of MMP-9 mRNA and TIMP-1 mRNA in HaCaT cells．Results：With the UVB irradiation accumulated, There was a decrease in the proliferation of HaCaT cells and an increase in the inhabation ratio, and there were significant differences in the values of OD (P < 0.05).The expression levels of MMP-9 mRNA increased,Comparative differences between groups were statistically significant(P<0.05).The expression levels of TIMP-1 decreased,There were significant differences in compare with0 mJ/cm2(P<0.05). Conclusion：UVB irradiation can induce the injure and apoptosis of HaCaT cells , MMP-9 and TIMP-1 maybe have some role in the pathogenesis of skin photoaging．

Key words: ultraviolet rays, keratins, photic stimulation, skin aging, matrix metalloproteinase 9, tissue inhibitor of metalloproteinase-1