天津医药

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膜联蛋白A5在人宫颈鳞癌不同分化型的表达及临床意义

王立平1,李 欣2   

  1. 1. 承德医学院附属医院
    2. 承德医学院
  • 收稿日期:2011-07-19 修回日期:2012-02-02 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 李 欣

Clinical significance of ANXA5 expression in human uterine cervical carcinoma of different pathological differentiation

  • Received:2011-07-19 Revised:2012-02-02 Published:2012-07-15 Online:2012-07-15

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摘要： 目的：检测膜联蛋白A5在临床不同分化细胞型中的表达差异，从而探讨膜联蛋白A5作为宫颈鳞癌组织分化标志物的可行性。方法：将收集的宫颈鳞癌组织HE染色后分为高分化型12例，中分化型25例，低分化型15例。用RT-PCR的方法检测RNA水平膜联蛋白A5的表达情况，以GAPDH为内参，对膜联蛋白A5 RNA的表达进行半定量检测；用Western Blot法检测蛋白水平膜联蛋白A5的表达，以β-actin为内参，进行蛋白表达的半定量检测；用免疫组织化学法对膜联蛋白A5在细胞内的定位以及表达量进行检测。计量资料组间结果采用t检验进行两两比较。结果：高分化型宫颈鳞癌组织中膜联蛋白A5的表达显著低于中、低分化组（P<0.05）；膜联蛋白A5在中、低分化的宫颈鳞癌组织中表达显著增高，但此两组之间差异无显著性（P>0.05）。结论：膜联蛋白A5具有作为临床宫颈癌细胞分化标志物的潜在可能。

关键词: 膜联蛋白A5, 细胞分化, 宫颈鳞癌, 表达

Abstract: [ABSTRACT] Objective: To study the possibility of ANXA5 as a differentiation type marker of uterine cervical carcinoma by examining expression of ANXA5 in the three kinds of pathology differentiation. Methods: All the specimens are classified as well-differentiated，intermediate-differentiated and poor-differentiated groups according to HE stainning. RT-PCR method was used to determine the ANXA5 mRNA expression; Western Blot method and immunohistochemistry were employed to determine the ANXA5 protein expression. The results between any two groups were analysed by t test. Results: ANXA5 expression in poor-differentiated and intermediate-differentiated groups increased drastically while the expression in well-differentiated group only lightly increased, which has significant difference with the other two groups（P<0.05）. Conclusion: ANXA5 maybe a potential marker for the differentiation types of uterine cervical carcinoma.

Key words: annexin A5, cell differentiation, uterine cervical carcinoma, expression