天津医药

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链球菌M蛋白同源角蛋白多肽在银屑病发病中的作用

高萃苹1,张理涛2,于广新2,宋蒙蒙2   

  1. 1. 天津医科大学
    2. 天津市中医药研究院附属医院
  • 收稿日期:2013-03-06 修回日期:2013-04-25 出版日期:2013-06-15 发布日期:2013-06-15
  • 通讯作者: 高萃苹

Role of Streptococcal M Protein Homologous Keratin Polypeptide in the Pathogenesis of Psoriasis

GAO Cuiping 1,ZHANG Litao 2,YU Guangxin 2,SONG Mengmeng 2   

  1. 1. Tianjin Medical University
    2. Affiliated Hospital of Tianjin Academy of Chinese Medicine
  • Received:2013-03-06 Revised:2013-04-25 Published:2013-06-15 Online:2013-06-15
  • Contact: GAO Cuiping

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摘要： 【摘要】目的  探讨与M蛋白同源的角蛋白肽段在银屑病发病中的作用。方法  人工合成与M蛋白同源的角蛋白肽段，氨基酸残基序列分别为ALEEAN、LKKDVDC、GLRRVLDE、RLKYENELA。4组合成同源肽段与银屑病患者和健康人（健康组）外周血单核细胞（PBMCs）共同培养。双抗体夹心ELISA法检测细胞因子白细胞介素（IL）-2、IL-4和γ干扰素（IFN-γ）的产生情况，MTT法检测PBMCs增殖情况。将合成同源肽段与角质形成细胞（HaCat）共培养，MTT法检测HaCat增殖情况，并对结果进行分析。结果  合成同源肽段ALEEAN和LKKDVDC均能刺激斑块型银屑病患者PBMCs增殖，IL-2、IL-4、IFN-γ分泌水平均显著高于健康组。在同源肽段GLRRVLDE、RLKYENELA中未检测到3种因子的表达。合成同源肽段ALEEAN、LKKDVDC、GLRRVLDE与角质形成细胞生长因子（KGF）共培养能促进HaCat增殖（均P＜0.05）。结论  与M蛋白同源的某些角蛋白多肽可引起银屑病患者PBMCs和HaCat增殖，可能在银屑病，尤其是在β-溶血型链球菌感染后的银屑病皮损的维持与加重中起一定作用。

关键词: 银屑病, 链球菌属, 溶血, 角蛋白细胞, 细菌蛋白质类, 血蛋白质类, 单核细胞, 白细胞介素2

Abstract: [Abstract]   Objective   To study the role of the keratin peptides homologous with M protein in the pathogenesis of psoriasis. Methods   Keratin peptides homologous to M protein were synthetized chemically. The sequences of amino acid residues for these peptides were ALEEAN, LKKDVDC, GLRRVLDE and RLKYENELA, respectively. The levels of interleukin (IL)-2, IL-4 and interferon (IFN)-γ were detected by double-antibody sandwich ELISA assay. The proliferation of peripheral blood mononuclear cells (PBMCs) was detected by MTT method. The keratinocytes (HaCaT) were co-cultured with synthetic peptides. The proliferation of HaCaT was detected by MTT method. Results   The expression levels of IL-2, IL-4 and IFN-γ were significantly higher in psoriatic PBMCs cultured with synthetic ALEEAN and LKKDVDC than those in normal PBMCs. These three cytokines were not detected in the cell culture supernatants of homologous peptides GLRRVLDE and RLKYENELA. The proliferation of HaCaT was promoted in synthetic peptides ALEEAN, LKKDVDC and GLRRVLDE co-treated with keratinocyte growth factor (KGF) (P < 0.05). Conclusion    The keratin peptides homologous with M protein could promote proliferation of PBMCs and HaCat in patients with plague psoriasis, which may play a certain role in the pathogenesis of psoriatic aggravation and maintenance after hemolytic streptococcus infection.

Key words: Psoriasis, Streptococcus, haemolysis, keratinocytes, bacterial proteins, blood proteins, monocytes, 白细胞介素2