天津医药

• 实验研究 •    下一篇

黄芩苷下调 p-STAT3 诱导神经干细胞向神经元分化

崔猛1,冯世庆1,范宁建2,贾军2,周先虎2   

  1. 1. 天津医科大学总医院骨科
    2. 天津医科大学总医院
  • 收稿日期:2012-10-22 修回日期:2013-04-08 出版日期:2013-08-15 发布日期:2013-08-15
  • 通讯作者: 崔猛

Baicalin promotes neuronal differentiation of neural stem/progenitor cells through downregulating p-stat3

CUI Meng ,FENG Shiqing ,FAN Ningjian ,JIA Jun ,ZHOU Xianhu   

  1. Department of Orthopedics， General Hospital of Tianjin Medical University， Tianjin 300052， China
  • Received:2012-10-22 Revised:2013-04-08 Published:2013-08-15 Online:2013-08-15
  • Contact: CUI Meng

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摘要： 【摘要】 目的 观察黄芩苷促进神经干细胞（NSCs）体外向神经元分化过程中信号传导子与转录激活子(STAT)的磷酸化蛋白表达水平。 方法 从孕 14～ 15 d 的 SD 大鼠胚胎大脑皮质中分离 NSCs， 体外培养传代， 实验用第 3 代NSCs。 随机分为对照组， 低、中、高黄芩苷组（分别含黄芩苷 7.5、15、30 μmol/L）， 白血病抑制因子（LIF）+碱性成纤维细胞生长因子（bFGF）组和黄芩苷+LIF+bFGF组。 培养 6 d后， 细胞免疫荧光化学染色法观察各组中微管相关蛋白 2(MAP-2)、胶质纤维酸性蛋白（GFAP）表达水平。 培养 2 h与 6 d后， 用 Western blot方法观察各组中 STAT3 蛋白磷酸化水平。 结果 黄芩苷可使NSCs 中 MAP-2表达增加， GFAP表达减弱； LIF+bFGF可促进 NSCs 中 GFAP表达增加； 黄芩
苷可抑制 LIF+bFGF引起的 NSCs 中 GFAP的表达增加。 黄芩苷可下调 NSCs 中 STAT3 蛋白磷酸化水平； LIF+bFGF可上调 NSCs 中 STAT3 蛋白磷酸化水平； 黄芩苷可抑制 LIF+bFGF 引起的 NSCs 中 STAT3 蛋白磷酸化水平的上调（P＜0.05）。 结论 黄芩苷可诱导 NSCs 向神经元分化并抑制其向星形胶质细胞分化， 该作用可能经下调 NSCs 中 STAT3的磷酸化水平来实现。

关键词: 黄芩苷, 信号传导, 转录, 遗传, 转录因子, 白血病, 干细胞, 神经系统

Abstract: [Abstract]  Objective  To observe the role of baicalin on the expression of phosphorylated protein of signal transducers and activators of transcription signaling proteins (STATs) during the process that neural stem cells (NSCs) differentiating into neurons. Methods  NSCs were isolated from the embryonic cerebral cortex of the 14-15-day pregnant SD rats, which were cultured and passaged in vitro. The 3rd generation of NSCs was used in the experiment. NSCs were randomized into natural differentiation control group, three different doses of baicalin groups (7.5 μmol/L, 15 μmol/L and 30 μmol/L), leukemia inhibitory factor (LIF) + basic fibroblast growth factor (bFGF) group and baicalin + LIF + bFGF group. After 6 d culture in vitro, the immunohistochemical method was used to observe the expressions of microtubule-associated protein 2(MAP-2) and glial fibrillary acidic protein (GFAP) in different groups. The expression levels of phosphorylation protein of STAT3 in NSCs were detected by Western blotting method after 2 h and 6 d of culture. Results The expression of MAP-2 in NSCs was increased by baicalin, but the expression of GFAP in NSCs decreased. The expression of GFAP in NSCs was enhanced in LIF+bFGF group, which was inhibited by baicalin + LIF + bFGF. The phosphorylation level of STAT3 in NSCs was downregulated by baicalin, but the phosphorylation level of STAT3 was upregulated in LIF+ bFGF group. The upregulated phosphorylation level of STAT3 was inhibited in baicalin + LIF + bFGF group(P＜0.05). Conclusion   Baicalin can induce NSCs to differentiate into neurons, which may be caused by the downregulation of the phosphorylation level of STAT3 in NSCs.

Key words: Baicalin, signal transduction, transcription, genetic, transcription factors, leukemia, stem cell, 神经系统