天津医药

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芪杞参颗粒对小鼠的细胞免疫和体液免疫功能的影响

李宏1,戴学文2,房志仲2,高卫真3   

  1. 1. 天津医科大学第二医院药学部
    2. 天津医科大学药学院药剂学教研室
    3. 天津医科大学基础医学院药理学教研室
  • 收稿日期:2013-04-26 修回日期:2013-05-29 出版日期:2013-09-15 发布日期:2013-09-15
  • 通讯作者: 李宏

he Influence of Qiqishen Granules on the Cellular and Humoral Immune Function

LI Hong 1,DAI Xue wen2,FANG Zhi zhong3,GAO Wei zhen4   

  1. 1. Department of Pharmacy, The Second Hospital of Tianjin Medical University
    2. Department ofPharmacy, School of Pharmacy, Tianjin Medical University
    3. Department of Pharmacy, School of Pharmacy, Tianjin Medical Universit
    4. Department of Pharmacology, Basic Medical CollegeTianjin Medical University
  • Received:2013-04-26 Revised:2013-05-29 Published:2013-09-15 Online:2013-09-15
  • Contact: LI Hong

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摘要：

【摘要】 目的 研究芪杞参颗粒对小鼠的细胞免疫和体液免疫功能的影响。方法??? 6周龄昆明种小鼠分空白对照组、芪杞参颗粒高、中、低剂量组，小鼠腹腔注射鸡红细胞混悬液，观察芪杞参颗粒对小鼠腹腔巨噬细胞吞噬功能的影响；制备绵羊红细胞(SRBC)，观察血球凝集程度，测定血清溶血素；以靶细胞(YAC-1细胞)与脾细胞(效应细胞)的反应检测芪杞参颗粒对小鼠自然杀伤（NK）细胞活性的影响；采用淋巴细胞转化法观察芪杞参颗粒对细胞免疫的影响；计算小鼠胸腺质量/体质量及脾脏质量/体质量为脏器系数观察芪杞参颗粒对小鼠脏器的影响。结果??? 与对照组比较，芪杞参颗粒显著增加小鼠腹腔巨噬细胞吞噬功能；对小鼠体液免疫功能有一定的增强作用；可增强小鼠NK细胞活性；对刀豆蛋白（Con）A诱导下的小鼠淋巴细胞转化有增强作用；对脏器系数没有显著的影响。结论??? 芪杞参颗粒具有明显的免疫调节作用，预示其有良好的应用前景。

关键词: 芪杞参颗粒, 免疫, 细胞, 体液免疫, 吞噬作用, 溶血素蛋白质类, 杀伤细胞, 天然

Abstract:

[Abstract]   Objective   To study the influence of qiqishen granules on the cellular and humoral immune functions in
model mice.  Methods   Six-week-old mice were divided into control group and qiqishen granule (high, medium and lowdose) groups. The suspension of chicken red blood cells was injected into the mouse abdominal cavity. The influence of qiqishen granules on the phagocytic function of the macrophages in mouse abdominal cavity was observed. The sheep red blood cells (SRBC) were prepared. The blood corpuscle coagulation was observed, and the serum hemolysin was detected. The activity of the mouse natural killer (NK) cells were detected by the interaction between the target cell (YAC-1) and spleen cell (the response cell). The influence of qiqishen granules on the cellular immunity was detected by the lymphocyte transforming assay. The influence of qiqishen granules on organ/body weight ratio was measured by calculating the thymus/body and spleen/body ratio.    Results    Compared with the control，qiqishen granules significantly improved phagocytic function of the macrophages in mouse abdominal cavity. The humoral immune function was also improved in mice. The activity of NK cell was enhanced by qiqishen granules, which also enhanced the lymphocyte transforming induced by the concanavalin (Con) A. There was no significant influence in the organ/body weight ratio.   Conclusion   The qiqishen granules could increase the immunomodulating effect, indicating it would have a good using prospect．

Key words: qiqishen granules, immunity, cellular, humoral immunity, phagocytosis, hemolysin proteins, killer cells, 天然