天津医药

• 论著 • 上一篇    下一篇

阿片生长因子抑制乳腺癌细胞生长的研究

邢恩鸿   

  1. 承德医学院附属医院中心实验室
  • 收稿日期:2010-04-07 修回日期:2010-09-20 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 邢恩鸿

The growth inhibitory effect of OGF on human breast carcinoma cells in vitro

enhong xing   

  • Received:2010-04-07 Revised:2010-09-20 Published:2011-01-15 Online:2011-01-15
  • Contact: enhong xing

PDF (PC)

2449

摘要： 摘要 目的：探讨阿片生长因子（OGF）对MCF-7人乳腺癌细胞生长的影响。方法:通过细胞计数、MTT实验、FCM细胞周期分析观察OGF对MCF-7细胞生长的影响。结果：细胞计数显示，OGF处理组细胞数明显低于OGFr拮抗剂naloxone处理组及空白对照组（P<0.01），而后两组细胞数差异无显著性（P>0.05）；MTT实验显示，OGF处理组细胞增殖抑制率明显高于naloxone处理组、OGF联合naloxone处理组及空白对照组（P<0.01），而后两组差异无显著性（P>0.05）；OGF处理组MCF-7细胞G0/G1期分数明显高于naloxone处理组及空白对照组（P<0.01）。结论：OGF能抑制MCF-7人乳腺癌细胞的生长。OGFr拮抗剂能阻断这种作用。OGF阻断细胞周期于G0/G1期。

关键词: OGF, OGFr, MCF-7细胞, 受体, 生长因子, 细胞周期

Abstract: Abstract Objective：To investigate the growth inhibitory effect of OGF and the opioid antagonist naloxone on MCF-7 human breast carcinoma cells. Methods：Cell counting、MTT essays and cell cycle distribution analysis by flow cytometry were used to detect the inhibiting growth effect of OGF on MCF- 7 cells. Results: Cell counting showed that the cell concentration reduced markedly in OGF group than other groups（P<0.01）. No difference was found between naloxone and control groups（P>0.05）. MTT essays indicated that the inhibiting rate of cell growth of OGF group was significantly higher than that in naloxone and OGF/naloxone groups (P<0.01). There was no significant difference among naloxone, OGF/ naloxone and control groups（P>0.05）. Flow cytometry results showed that PI was lower in OGF group than in other groups（P<0.01）, and no significant difference was found between naloxone and control groups（P>0.05）.Conclusions: OGF could inhibit the growth of MCF-7 cells in vitro and the growth inhibitory effect of OGF can be blocked by naloxone. The cell cycle was obstructed by OGF in G0/G1 phase.

Key words: OGF, OGFr, MCF-7 human breast carcinoma cells, receptor, growth factor, cell cycle