天津医药

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天津地区2008年至2009年婴幼儿诺如病毒的分子流行病学特征

王瑞雪1,2,林书祥3,彭林3,王维3   

  1. 1. 山西省人民医院
    2. 天津市儿童医院检验科 马慧
    3. 天津市儿童医院
  • 收稿日期:2010-02-02 修回日期:2010-04-21 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: 彭林

Characterization of molecular epidemiology of Norovirus in infants and young children in Tianjin ,2008-2009

  • Received:2010-02-02 Revised:2010-04-21 Published:2010-11-15 Online:2010-11-15

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摘要： 【摘要】 目的：了解2008年8月到2009年7月天津地区婴幼儿诺如病毒感染情况并对诺如病毒进行基因分型及分析。方法：收集2008年8月到2009年7月天津儿童医院门诊和住院病毒性腹泻的粪便标本。用反转录-聚合酶链反应（RT-PCR）进行检测。测序后使用Bioedit和 DNAstar基因分析软件与Genbank中的参考株进行同源性分析并绘制系统进化树。结果：在540例腹泻标本中诺如病毒阳性的标本为174 例。诺如病毒的阳性率为 32.22%. 经序列分析所有诺如病毒阳性标本均为GⅡ-4型。结论：诺如病毒是天津地区婴幼儿病毒性腹泻的主要病原体之一。GⅡ-4型是主要流行株。

关键词: 诺如病毒, 基因型, 逆转录聚合酶链反应, 腹泻

Abstract: 【Abstract】:Object: To understand the status of Novorirus infection in infants and children in Tianjin area , and study the genotype and the genetic identity of Norovirus . Methods : The fecal specimens from infants and young children with viral gastroenteritis were collected from Tianjin Children hospital from August 2008 to July 2009 .The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR). The amplied genes were the sequenced and compared with the Norovirus sequences deposited in GeneBank. By DNAstar and Bioedit software to evaluate sequence homology and generated the phylogenetic tree .Results: NV was detectable in 174 cases out of the 540 specimens. The positive rate was 32.22% .Nucleotide sequence analysis showed that the NrV strains all belonged to GⅡ-4 . The sequences of specimens were closer to the viral sequence obtained from Beijing . Conclusion: NV is one of the important diarrhea pathogens of viral gastroenteritis in infants and young children in Tianjin area and the GⅡ-4 is the major prevalent strain.

Key words: norovirus, genogroup, RT-PCR, diarrhea