天津医药

• 论著 •    

体内外高糖对血管生成素样蛋白3表达的影响

肖亚雄1,王继红1,孔渝菡2   

  1. 1. 重庆医科大学
    2. 重庆医科大学附属第一医院
  • 收稿日期:2010-03-03 修回日期:2010-04-19 出版日期:2010-08-15 发布日期:2010-08-15
  • 通讯作者: 王继红

Effects of high glucose on angiopioetin-like protein 3 expression in vitro and in vivo

  • Received:2010-03-03 Revised:2010-04-19 Published:2010-08-15 Online:2010-08-15

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摘要： 摘要 目的 观察人体血液中葡萄糖浓度对血管生成素样蛋白3(ANGPTL3)蛋白质表达的影响，以及体外高浓度葡萄糖对正常人肝细胞株（L02）ANGPTL3 mRNA及蛋白质表达的影响，探讨ANGPTL3与糖尿病发生的关系。方法 以人血清和L02细胞作为研究对象。从临床采集健康人（对照组）、高血糖患者（实验组）空腹血清各10例，各组男女比例均为5：5。用ELISA法测定血清ANGPTL3蛋白质浓度、全自动生化仪测定血糖浓度。将L02细胞细胞置于含5.6 mmol/L，7.1 mmol/L，11.2 mmol/L，20.0 mmol/L葡萄糖的培养基中培养，以葡萄糖浓度5.6 mmol/L为对照组，用RT-PCR方法检测L02细胞ANGPTL3的mRNA表达量，并用Western Blot方法检测其蛋白质表达量。结果 高血糖组血清ANGPTL3蛋白质含量较健康组明显升高，差异有统计学意义（P<0.05）。体外培养基中高糖可以促进L02细胞ANGPTL3基因和蛋白质表达，与对照组比较差异有统计学意义（P<0.05）；结论 体内外高糖环境均可以上调ANGPTL3的表达。ANGPTL3高表达可能与糖尿病发生存在一定关联。

关键词: 血管生成素样蛋白3, 糖尿病, 葡萄糖, 高血糖

Abstract: Abstract:Objective To observe the effects of high glucose on angiopioetin-like protein 3(ANGPTL3) expression in vivo and in vitro .To explore the relationship and of ANGPTL3 with the diabetes mellitus （DM）. Methods Take human serum and L02 as research subjects including 10 cases healthy human serum as control group（male : famale=5:5）and 10 cases of hyperglycemia serum as experimental group (M:F=5:5) from clinic. L02 cells were cultured separately in mediums containing 5.6，7.1，11.2 and 20mmol/L glucose，among which 5．6 mmol／L glucose group was taken as contro1；The relative level of ANGPTL3 protein in serum was measured by ELISA .The relative level of ANGPTL3 mRNA expression was detected by reverse transcription-polymerase chain reaction(RT-PCR). The relative level of ANGPTL3 protein in L02 was measured by Western Blot. Results The concentration of ANGPTL3 protein in hyperglycemia group was obviously higher than that of ctrol group(P<0.05).High concentration of glucose in medium could stimulate the expresion of ANGPTL3 mRNA and protein in cultured L02 cells . The differences were significant (P<0.05).Conclusion high glucose can upregulate ANGPTL3 expression both in vtro and in vivo. The expression of ANGPTL3 might by related to DM.

Key words: angiopioetin-like protein 3（ANGPTL3）, diabetes mellitus（DM）, glucose, hyperglycemia