天津医药

• 论著 •    

血红素加氧酶-1启动子基因多态性与高血压的关系

陈树涛,丛洪良   

  1. 天津市胸科医院
  • 收稿日期:2011-03-07 修回日期:2011-09-05 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 陈树涛

The relationship between heme oxygenase-1 promoter polymorphism And hypertension

  • Received:2011-03-07 Revised:2011-09-05 Published:2012-01-15 Online:2012-01-15
  • Contact: Shu-Tao CHEN

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摘要： 摘要：目的： 探讨血红素加氧酶-1（HO-1）基因启动子区域的（GT）n双核苷酸重复序列多态性与天津地区原发性高血压（EH）的关系并分析该多态性与血浆炎症因子及胆红素水平的关联性。方法： 选择102例EH病例及134例正常对照组作为研究对象，采用聚合酶链反应（PCR）方法扩增染色体DNA中含HO-1基因相应的DNA片段，聚丙烯酰胺凝胶电泳（PAGE）技术分析基因型。自动生化分析仪分析血浆胆红素含量。ELISA法分析血清白介素-10及肿瘤坏死因子-?的含量。结果：高血压组血浆胆红素、TNF-α、白介素-10水平显著高于对照组，差异有统计学意义（P?0.05）。HO-1重复≥32次长等位基因频率在EH组的分布明显高于健康对照组，差异有非常显著意义（P?0.01）。HO-1长等位基因携带者血浆胆红素IL-10水平低于短等位基因携带者，而TNF-α水平则高于短等位基因携带者。但只有胆红素水平有显著性差异。结论： 血浆胆红素、TNF-?及抗炎因子IL-10水平与高血压的发病相关。HO-1基因启动子区域的（GT）n双核苷酸重复多态性可能与EH人群的易感性相关。HO-1长等位基因携带者患高血压的危险性增大。

关键词: 原发性高血压（EH）, 血红素加氧酶（HO-1）, 基因多态性

Abstract: Background: Heme oxygenase-1 is a kind of antioxidative enzyme. A dinucleotide GT repeat in promoter region of Human HO-1 gene shows a length polymorphism that may modulates the level of gene transcription. Therefore this polymorphism phenomenon may associate with the development of essential hypertension. Objective: (1) To investigate the relationship between the length polymorphism of GT repeat in HO-1 promoter area and essential hypertension.(2) To observe if this polymorphism will affect the levels of bilirubin, TNF-? and IL-10 in plasma. (3) To evaluate the effect of the genotypes in the plasma levels of bilirubin, TNF-? and IL-10. Methods: The HO-1 gene GT dinucleotide repeat polymorphism in promoter area was evaluated in 102 EH patients and 134 healthy controls. The genotypes were analyzed by PCR and polyacrylamide gel electrophoresis. Plasma levels of TNF-? and IL-10 were obtained by ELISA methods. Bilirubin levels were measured by automatic chemistry analyzer. Results: (1) The plasma levels of bilirubin, TNF-? and IL-10 in EH group were higher than in control group. (the levels of bilirubin, TNF-? and IL-10 in EH group were 12.53?4.41umol/L; 2.22?0.57ng/ml; 40.87?5.53pg/ml respectively VS control group: 11.46?5.53umol/L; 1.97?0.47ng/ml; 38.17?4.46pg/ml p?0.05 ) (2) The long allele with ≥32 (GT)n repeats (L allele) was found more frequently in hypertension group(26.5% vs 14.6% X2 =10.42, p?0.05). (3) There was no significant difference to be found between the genotypes and the plasma levels of TNF-? and IL-10.The plasma bilirubin levels in the cases carrying with long allele were higher than the cases carrying with short allele. Conclusions: The long allele of HO-1 genotype may be associated with the development of essential hypertension. The levels of bilirubin, TNF-? and IL-10 in plasma may affect the development of EH .

Key words: hypertension, heme oxygenase-1, polymorphism