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CagA的表达对慢性胃炎Th细胞亚群分化的影响

谢兆兰   

  1. 武汉市武昌医院
  • 收稿日期:2013-01-04 修回日期:2013-05-20 出版日期:2013-08-15 发布日期:2013-08-15
  • 通讯作者: 谢兆兰

The Influence of CagA on The Differentiation of Helper T Lymphocytes in Chronic Gastritis

Zhao-Lan XIE   

  • Received:2013-01-04 Revised:2013-05-20 Published:2013-08-15 Online:2013-08-15
  • Contact: Zhao-Lan XIE

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摘要： 目的 探讨幽门螺杆菌CagA的表达对慢性胃炎患者胃黏膜Th细胞亚群分化的影响。 方法 80例慢性胃炎患者，ELISA法测定血清CagA 抗体，根据结果将慢性胃炎患者分为CagA阳性和阴性组，均取患者胃黏膜行病理检查及行PCR、Western blot检测核转录因子TBX21、GATA-3、FoxP3和Rorγt的表达水平。结果 CagA抗体阳性组炎症程度显著高于阴性组；2组间Hp菌密度差异无统计学意义。CagA抗体阳性组TBX21、Rorγt的转录及蛋白表达水平较阴性组显著下调，而GATA-3、FoxP3的转录及蛋白表达水平较阴性组显著上调。结论 慢性胃炎CagA 阳性患者的胃黏膜炎症程度更重，但机体却不能有效清除Hp在胃黏膜中的定植，其原因与可能存在Th0向Th2/Treg的分化偏倚有关。

关键词: 螺杆菌, 幽门, 胃炎, T淋巴细胞, 辅助诱导, GATA3转录因子, 细胞毒素相关蛋白A, TBX21

Abstract: Objective   To evaluate the influence of cytotoxin associated gene A to the differentiation of helper T lymphocytes in chronic gastritis. Methods   Eighty patients with chronic gastritis were in roll, the serum antibody against CagA was detected through ELISA. We divided the patients into CagA-positive and CagA-negative groups. Pathologic changes in gastric mucus were respectively analyzed. In addition, the expressions of nuclear transcriptive factors in gastric mucus reflecting the differentiation of Th subsets including TBX21、GATA-3、FoxP3 and Rorγt were further detected. Results   The gastric inflammation in CagA-positive group was more serious than that in CagA- negative one. However, the statistical difference of Hp density between them was not observed. The expressions of GATA-3 and FoxP3 were much higher while those of TBX21 and Rorγt much lower in CagA-positive group than that in CagA-negative one. Conclusions   Although the inflammation in gastric mucus of CagA-positive patients was relatively more serious, Hp can not be effectively eliminated，which may relate to the differentiation of Th0 into Th2/Treg cell.

Key words: Helicobacter pylori, gastritis, T-lymphocytes, helper-inducer, GATA3 transcription factor, CagA, TBX21, TBX21