天津医药

• 实验研究 •    

金芪降糖片对胰岛素抵抗大鼠肝脏SREBP－1cmRNA表达的影响

孙楠1,冯凭2,高志红3,崔景秋3   

  1. 1. 天津医科大学总医院；新疆自治区人民医院内分泌科
    2. 天津医科大学总医院
    3.
  • 收稿日期:2009-12-10 修回日期:2010-03-26 出版日期:2010-08-15 发布日期:2010-08-15
  • 通讯作者: 孙楠

The effect of Jinqi hypoglycemic table on expression of SREBP-1cmRNA in the liver of insulin-resistant rats

  • Received:2009-12-10 Revised:2010-03-26 Published:2010-08-15 Online:2010-08-15
  • Contact: SUN Nan

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摘要： 【摘要】目的 观察金芪降糖片对胰岛素抵抗（IR）大鼠肝脏固醇调控元件结合蛋白-1c（SREBP－1c）mRNA表达的影响。方法 高脂饲料喂养8周建立IR大鼠模型，将IR大鼠随机分为2组：高脂饮食（HF）组和高脂饮食＋金芪降糖（HF＋JQ）组，继续喂养6周后比较胰岛素敏感指数（ISI）、血脂、血清肿瘤坏死因子-α（TNF-α）、瘦素等指标和肝脏SREBP－1c mRNA的表达。结果 与HF组相比，HF＋JQ组大鼠血脂及血清瘦素、TNF-α均明显降低，ISI升高，肝脏SREBP－1c mRNA表达降低[(0.92±0.05 vs 0.75±0.06)，P<0.01]。结论 金芪降糖能抑制IR大鼠肝脏SREBP－1c的过度表达，有效改善IR。

关键词: 胰岛素抵抗, 固醇调控元件结合蛋白-1c, 瘦素, 肿瘤坏死因子－α, 降血糖药（中药）

Abstract: The effect of Jinqi hypoglycemic table on expression of SREBP-1cmRNA in the liver of insulin-resistant rats SUN Nan，CUI Jing-qiu，GAO Zhi-hong, FENG Ping, The General Hospital Of Tianjin Medical University，Tianjin 300052，China 【Abstract】Objective To investigate the efficacy of Jinqi hypolgycemic tablet on expression of SREBP-1c mRNA in the liver of insulin-resistant rats. Methods Established a rat model of insulin resistance (IR) by feeding high-fat diet for 8 weeks, then randomly divided the insulin-resistant rats into two groups: HF group (n＝9) that was fed with high-fat diet for another 6 weeks,，HF＋JQ group (n＝9) that was fed with high-fat diet and Jinqi hypolgycemic tablet for 6 weeks. Measured blood glucose, serum insulin, lipid concentrations and serum leptin, serum TNF-α. Semi-quantitative RT-PCR was used to detect the expression of SREBP-1c mRNA in the liver of rats at the end point. Results Compared with the HF group, serum insulin, OGTT 2h glucose, serum lipid, serum leptin and TNF-αconcentrations in HF＋JQ group were decreased obviously, and insulin sensitive index（ISI）was higher. In addition, , the expression of SREBP-lc in the liver of HF＋JQ group was significantly reduced compared with HF group[(0.75±0.06 vs 0.92±0.05)，P<0.01]. Conclusion Jinqi hypolgycemic tablet can inhibit the expression of SREBP-1c mRNA in the liver of high-fat-fed rats and improve insulin resistance . 【Key words】 Insulin resistance, SREBP－1c, leptin, TNF-α, Hypoglycemic drugs (traditional Chinese medicine

Key words: Insulin resistance, SREBP－1c, leptin, TNF-α, Hypoglycemic drugs (traditional Chinese medicine)