天津医药

• 实验研究 •    

先天性甲状腺功能减退症大脑蛋白质双向电泳分析

刘春蓉1,于保国2,杨术旺2,刘燕青2,刘亚敏2,张永亮2   

  1. 1. 武警医学院基础部病理学教研室
    2.
  • 收稿日期:2010-01-20 修回日期:2010-02-10 出版日期:2010-06-15 发布日期:2010-06-15
  • 通讯作者: 刘春蓉

Differential proteomics analysis of cerebral cortex in experimental congenital hypothyroidism neonatal rat.

  • Received:2010-01-20 Revised:2010-02-10 Published:2010-06-15 Online:2010-06-15

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摘要： 目的：比较新生正常大鼠和CH大鼠大脑蛋白质表达的差异，为探讨先天性甲状腺功能减退症（CH）致脑发育障碍发病机制提供线索。方法：制作CH仔鼠动物模型，于出生时称体质量后处死仔鼠，取大脑皮质，应用双向电泳（2-DE）技术分析正常仔鼠与CH仔鼠大脑蛋白质表达的差异情况。放射免疫分析法检测2组大鼠血清游离三碘甲腺原氨酸（FT3）、游离甲状腺素（FT4）水平。结果：CH组仔鼠体质量、FT3、FT4水平均低于正常组仔鼠。建立了稳定的正常仔鼠与CH仔鼠大脑皮质2-DE图谱，发现2组共有8个蛋白质点存在差异。与正常仔鼠相比，CH仔鼠大脑有3个蛋白质点量上调，2个蛋白质点量下调，有3个蛋白质点仅出现于CH仔鼠组。结论：CH仔鼠大脑差异蛋白表达可能与CH致脑发育障碍的发生、发展密切相关。

关键词: 先天性甲状腺功能减退症, 大鼠, 大脑皮质, 双向电泳

Abstract: Objective:To find the expressive proteomics changes in experimental congenital hypothyroidism neonatal rat cerebral cortex，, which provide valuable clues to clarify the pathogenesis of CH-induced brain developmental disorders. Methods:Neonatal hypothyroidism rat was induced by administation of propylthiouracil （PTU） solution to the dams by gavage（1%PTU，2.5ml/d） on embryonic day 13.Cerebral cortex were collected from the control and hypothyroidism neonatal rats.2-DE analysed protein expression diversities between the euthyroid and hypothyroidism neonatal rat cerebral cortex.RIA analysed serum FT3,FT4 level of each groups. Results:Compared with control group,body weight and serum FT3,FT4 level in CH neonatal rat were lower.Stable 2-DE maps of normal and CH neonatal rat were constantly obtained.Maps were analyzed by software.Eight differential protein spot were found,three differential protein spot were highly expressed in CH group,two differential protein spot were highly expressed in control group,three protein spot present only in CH group.Conclusions:Differential protein expression in CH neonatal rat cerebral may play critical roles in CH.

Key words: congenital hypothyroidism, rat, cerebral cortex, 2-DE