天津医药

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电化学发光免疫法检测促甲状腺素受体抗体的临床价值

冯学民,刘尧娟   

  1. 天津市第一中心医院核医学科
  • 收稿日期:2011-09-26 修回日期:2011-12-22 出版日期:2012-08-15 发布日期:2012-08-15
  • 通讯作者: 冯学民

Clinical value of electrochemiluminescence immunoassay for measuring Thyrotropin receptor antibody

  • Received:2011-09-26 Revised:2011-12-22 Published:2012-08-15 Online:2012-08-15

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摘要： 摘要 目的：评估全自动电化学发光免疫法(ECLIA)检测血清促甲状腺素受体抗体（TRAb）的临床价值。方法：使用ECLIA和放射受体法（RRA）检测150例Grave’病（GD）、56例桥本甲状腺炎（HT）、73例亚急性甲状腺炎（ST）和95例健康对照（HS）受试者血清TRAb，通过ROC曲线计算最佳cut-off值。结果：ECLIA 法cut-off值为1.85IU/L诊断GD的灵敏度和特异性分别为94.7%和91.6%，曲线下面积0.935，优于RRA法。结论： 全自动ECLIA 检测TRAb对于GD的诊断具有较高的灵敏度和特异性，特别是与其他甲状腺毒症的鉴别，代表将来TRAb检测的发展方向。 关键词：促甲状腺素受体抗体 电化学发光免疫法 Graves’病 ROC曲线

关键词: 促甲状腺素受体抗体, 电化学发光免疫法, Graves’病, ROC曲线

Abstract: Abstract 0bjective: We evaluated clinical value of fully automated electrochemiluminescence immunoassay (ECLIA) for measuring serum TRAb. Methods: Totally 150 Graves’disease（GD）, 56 Hashimoto’s thyroiditis（HT）,73 subacute thyroiditis(ST) and 95 healthy subjects(HS) were retrospectively evaluated by both ECILA and radioactive receptor assay(RRA). The optimal cut-off which was calculated by receiver operating characteristic (ROC).Results: The cut-off value of 1.85 IU/L from the ROC on the GD cases was chosen as the cut-off with 94.7% of sensitivity and 91.6% of specificity. The area under the ROC curve was 0.935. The new automated assay was superior to RRA. Conclusion: Results demonstrate that this fully automated system has a high sensitivity and speci?city for diagnosis GD, especially for identifying it from various forms of thyrotoxicosis.. This assay may represent the future technology for TRAb detection.

Key words: TSH receptor antibody, electrochemiluminescence immunoassay, Graves’disease, curve of receiver operating characteristic