天津医药

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尼莫地平对弥漫性轴索损伤大鼠脑水肿的影响

赵新亮1,王毅2,冯洁3,张建宁2   

  1. 1. 天津市永久医院
    2. 天津医科大学总医院
    3. 南开医院
  • 收稿日期:2012-02-03 修回日期:2012-05-11 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 王毅

The Study on Nimodipine on the Effect of Blood-brain Barrier of Mouse with Diffuse Axonal Injury

  • Received:2012-02-03 Revised:2012-05-11 Published:2012-07-15 Online:2012-07-15
  • Contact: Yi WANG

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摘要： 【摘要】 目的：评价尼莫地平对弥漫性轴索损伤（DAI）大鼠脑水肿的影响。 方法：将100只Wistar大鼠随机分为DAI组和治疗组，治疗组于造模后给予尼莫地平注射液腹腔注射，剂量为0.5mg/kg体重/d，分别于伤前和伤后6h、1d、3d、7d通过检测脑组织含水量反应脑水肿情况，通过观察伊文蓝（EB）含量反应BBB通透性变化。结果：DAI后脑组织含水量和脑组织EB含量呈先升高后下降的趋势，峰值分别出现在伤后1d和3d，DAI组和尼莫地平治疗组不同时间的变化趋势比较差异有统计学意义（P<0.05)，2组峰值比较差异有统计学意义（P<0.05)，尼莫地平治疗组较低。结论：使用尼莫地平可以有效控制弥漫性轴索损伤大鼠的血脑屏障通透性，减轻脑水肿。

关键词: 弥漫性轴索损伤, 尼莫地平, 脑水肿, 血脑屏障

Abstract: [Abstract] Objective：To observe the therapeutic effect of nimodipine in treatment of Diffuse axonal injury (DAI) . Methods：100 mouse with DAI were divided randomly into two groups. In treatment group nimodipine was injected intravenously for 7 days. In control group mouse receive the same treatment measures but no nimodipine was given. The brain water content and the EB content at every time point ( before DAI and 6h, 1d, 3d, 7d after DAI) were closely observed. Results：The climax of the brain water content came to at 1d after DAI, and one of the EB content came to at 3d after DAI. The proportion of the brain water content and the EB content at 6h, 1d, 3d, 7d after DAI in treatment group were significantly lower than the control group（P<0.05). Conclusion：Nimodipine decreases the permeability of BBB and has definitive effect for brain edema after DAI.

Key words: Diffuse Axonal Injury, Blood-brain Barrier, Edema, Nimodipine