天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (9): 920-924.doi: 10.11958/20170839

• 实验研究 • 上一篇    下一篇

β-七叶皂苷钠对颅脑创伤大鼠脑水肿的保护作用

刘宝虎 1，2，郭彤彤 1，江继鹏 2，陈旭义 2，边科砜 3，张赛 2△   

  1. 1 天津中医药大学（邮编 300193）；2 武警后勤学院附属医院脑科中心，武警部队脑创伤与神经疾病研究所，天津市神经创伤修 复重点实验室；3 武警后勤学院学员二旅
  • 收稿日期:2017-07-26 修回日期:2017-08-09 出版日期:2017-09-15 发布日期:2017-09-25
  • 通讯作者: 刘宝虎 E-mail:645358197@qq.com
  • 基金资助:
    国家重点研发计划

Neuroprotective effects of β-aescinate on brain edema in rat model of traumatic brain injury

LIU Bao-hu1,2, GUO Tong-tong1, JIANG Ji-peng2, CHEN Xu-yi2, BIAN Ke-feng3, ZHANG Sai2?   

  • Received:2017-07-26 Revised:2017-08-09 Published:2017-09-15 Online:2017-09-25
  • Contact: Bao-Hu LIU E-mail:645358197@qq.com

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摘要： 摘要：目的 探讨 β-七叶皂苷钠对颅脑创伤（TBI）大鼠脑水肿的保护作用。方法 筛选成年雄性 SD 大鼠 78 只，按随机数字表法分为假手术（Sham）组、颅脑创伤（TBI）组、β-七叶皂苷钠组，每组 26 只。Sham 组仅开骨窗不做 处理；TBI 组和 β-七叶皂苷钠组开骨窗后采用电子脑皮质损伤撞击仪（eCCI）建立大鼠 TBI 模型，其中 TBI 组只建模 但不干预，β-七叶皂苷钠组建立模型后即刻给予腹腔注射 β-七叶皂苷钠（5 mg/kg），每 24 h 重复给药 1 次。损伤后 48 h 采用改良神经功能缺损评分（mNSS）观察 TBI 神经功能改变，伤后 48 h 处死大鼠并取脑组织行 HE 染色（n=6）； 采用干/湿比重法测定脑组织含水量变化（n=10）；采用伊文思蓝染色（EB）检测血脑屏障（BBB）通透性（n=4）；采用免 疫印迹（Western-blot）法检测水通道蛋白 4（AQP4，n=6）的表达水平。结果 与 Sham 组比较，TBI 大鼠神经功能受 损，脑组织含水量增加，BBB 被破坏，AQP4 蛋白表达升高；而 β-七叶皂苷钠可改善 TBI 大鼠的神经功能受损程度， 降低脑组织含水量，改善 BBB 通透性，并显著降低 AQP4 表达水平（均 P＜0.05）。结论 β-七叶皂苷钠对 TBI 大鼠 有脑保护作用，可显著改善大鼠受损的神经功能，降低伤后脑水肿，改善 BBB 通透性，其机制可能与下调 AQP4 的表 达有关。

关键词: 七叶素, 颅脑损伤, 脑损伤, 水通道蛋白质 4, 脑水肿, β-七叶皂苷钠

Abstract: Abstract: Objective To explore the neuroprotective effects of β-Aescinate on brain edema in traumatic brain injury (TBI) mice. Method A total of 78 male SD rats (Sprague Dawley Rat) were randomly divided into three groups:sham-operation group (Sham), traumatic brain injury group (TBI), β-Aescinate group, with 18 rats in each. (n=26 per group). The Sham-operated animals were anesthetized and surgically prepared only,but were not induced by cortical contusion;TBI group and β-Aescinate group using eCCI establish TBI model after skullcap was carefully removed, TBI group only establish TBI model，but no deal with; The TBI model of β-Aescinate group was established, and thenβ-Aescinate (5 mg/kg body weight) via intraperitoneal injection,one times per 24 hours. Each of measurement was performed after 48 hours, Neurological function were evaluated by modified neurological severity scores (mNSS); SD rats were sacrificed at 24 h after TBI and stained using hematoxylin and eosin (H&E)(n=6); Additionally, the cerebral edema was evaluated from the water content of the brain tissue using the wet-to-dry weight ratio(n=10); and Evans Blue assay were performed to investigate the neurologic deficit, cer- ebral water content (n=8);and blood-brain barrier permeability (BBB) (n=4) ;and the of aquaporin 4 (AQP4) was measured by Western Blot assay(n=6).Result Compared with the Sham group, TBI group were shown to neurologic deficit ,and the brain water content and the of AQP4 were increased (all P<0.05).moreover，BBB permeability is destroyed. However, β-Aescinate could improve the neurological function, reduce the brain water content, and significantly decreased the of AQP4 compared with the TBI group (all P < 0.05) BBB permeability of each treatment group was also significantly improved (all P＜0.01). Conclusion These finding suggested that β-Aescinate could reduce cerebral edema and improve neurological outcome in SD rat after TBI to exert neuroprotection, through which reverses the high level of AQP4 protein.

Key words: escin, craniocerebral trauma, brain injuries, aquaporin 4, brain edema, β-aescinate