天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (6): 607-610.doi: 10.11958/j.issn.0253-9896.2015.06.008

• 实验研究 • 上一篇    下一篇

重型颅脑创伤大鼠后期脑水肿研究及牛磺酸转运体的作用研究

蔡英， 黄慧玲， 范维佳， 武俏丽， 李晓茜， 苏彦华， 温晓昶   

  1. 天津市环湖医院,天津市神经外科研究所， 天津市脑血管与神经变性重点实验室
  • 收稿日期:2015-01-08 修回日期:2015-03-02 出版日期:2015-06-15 发布日期:2015-06-10
  • 通讯作者: 蔡英 E-mail:caiying006@163.com
  • 基金资助:
    国家自然科学基金资助项目（30973089）； 天津市应用基础及前沿技术研究计划项目（10JCYBJC23200）； 天津市卫生局科技基
    金项目 （2012KR09）

Protection role of taurine transporter in rats brain edema followed severe traumatic head injury

CAI Ying, HUANG Huiling, FAN Weijia, WU Qiaoli, LI Xiaoqian, SU Yanhua, WEN Xiaochang   

  1. Tianjin Huanhu Hospital, Neurosurgery Institute of Tianjin,Tianjin Cerebrovascular and Neurodegeneration Key Laboratory
  • Received:2015-01-08 Revised:2015-03-02 Published:2015-06-15 Online:2015-06-10

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摘要： 目的 研究牛磺酸转运体(TauT)在牛磺酸调节重型颅脑创伤大鼠后期脑水肿中的作用。方法 将 40 只大鼠按随机数字表法分为 4 组:假手术组 （Sham 组）、 脑外伤组 （TBI 组）、 牛磺酸预防组 （Pre-Tau 组)和牛磺酸治疗组（Tau 组）。液压冲击法制作重型颅脑创伤大鼠模型。Pre-Tau 组和 Tau 组分别在造模前或后给予 120 g/L 的牛磺酸溶液， 其他两组给予等量的等渗生理盐水。7 d 后用干湿重法检测脑组织中脑水含量， 实时荧光定量 PCR 和 Western Blot 方法检测 TauT 和水通道蛋白(AQP4)的 mRNA 表达及蛋白含量。结果 与 Sham 组相比， TBI 组脑水含量、AQP4 的 mRNA 和蛋白表达水平均显著升高， TauT 的 mRNA 和蛋白表达水平显著降低。与 TBI 组比较， Pre-Tau 组和 Tau 组脑水含量、 AQP4 的 mRNA 和蛋白表达水平显著降低、 TauT 的 mRNA 表达明显升高； Tau 组 TauT 的蛋白表达明显升高； Pre-Tau 组 TauT 的蛋白表达有所升高， 但差异无统计学意义。AQP4 的 mRNA 和蛋白表达水平与脑水含量呈正相关 （r 分别为 0.621 和 0.457）， AQP4 的 mRNA 与蛋白表达亦呈正相关 （r=0.459）。结论 牛磺酸通过提高重型颅脑创伤大鼠后期 TauT 的表达水平， 降低脑水含量和 AQP4 的表达， 发挥神经元保护作用。

关键词: 牛磺酸, 脑损伤, 膜转运蛋白质类, 脑水肿, 水通道蛋白质 4, 大鼠, Sprague-Dawley, 牛磺酸转运体

Abstract: Objective To investigate the effect of taurine transporter in the process of protection of brain edema in rats with severe traumatic head injury. Methods A total of 24 Male Sprague-Dawley rats were randomly divided into 4 groups.Except the control rats (Group Sham), all other three groups were subjected to lateral fluid percussion head injury. The TBI(Traumatic brain injury) models (Group TBI) and surgical control rats (Group Sham) were injected with saline through caudal
vein after surgery, while the Taurine prevention and Taurine treatment models (Group Pre Tau and Group Tau) were injected with 120 g/L taurine solution before or after surgeries respectively. Water content in each brain, mRNA and protein expression of aquaporin 4 and taurine transporter in the injured rat brain hemispheres were all evaluated over the time course of the study (7 d) in each group. Results Compared with rats in Group Sham, water content in each brain increase, mRNA transcription and protein expression of AQP4 were both up regulated but the mRNA transcription and protein expression of TauT
were both down-regulated in rats in TBI group. Compared with rats in TBI group, brain water content, mRNA transcriptionand protein expression of AQP4 all decrease while mRNA transcription and protein expression of TauT all increase in rats in Pre tau and Tau groups. There is no statistical difference of TauT expression between rats in pre-tau group and Tau group.Conclusion Taurine exert its neuron protection role through draining water content from brain and down regulating expression of AQP4 but rising expression of TauT after TBI.

Key words: Taurine, brain injuries, membrane transport proteins, brain edema, aquaporin 4, rats, Sprague-Dawley, taurinetransporter transporter