天津医药

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左西孟旦对大鼠离体心脏缺血再灌注损伤的保护作用

于明懂,高春霖,吕国义,邓廼封   

  1. 天津医科大学第二医院麻醉科
  • 收稿日期:2010-04-06 修回日期:2010-05-26 出版日期:2011-09-15 发布日期:2011-09-15
  • 通讯作者: 高春霖

The Portective Efect of Levosimendan in STH-2 Cardioplegic SoIution on Ischemic/reperfusion Injury in IsoIated Rat Heart

  • Received:2010-04-06 Revised:2010-05-26 Published:2011-09-15 Online:2011-09-15

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摘要： 目的:观察含左西孟旦(1evosimendan，Levo)的停搏液对离体大鼠缺血再灌注心肌的保护作用。方法: 32只雄性Wisart大鼠随机分为4组(n=8)：L0、L00.3、L0.3、LG，在St.Thomas ’Ⅱ(STH-2)液中分别加入Levo 0μmol/L、0.03μmol/L、0.3μmol/L、0.3μmol/L+格列苯脲10μmol/L。利用改良的Langendorf离体鼠心灌注装置，平衡灌注30min后分别用25℃四种不同停搏液停搏，2h后重新灌注30min,观察对比各组停搏前后不同时间点心功能指标、再灌注末冠脉流出液中乳酸脱氢酶(LDH)和肌酸激酶(CK)含量的不同。结果：停搏前组间各指标无明显差异（P>0.05）, 恢复灌注后,L0.3组各心功能指标恢复率明显高于其他各组(P<0.01或P<0.05),再灌注末CPK和LDH漏出量明显低于其余各组(P<0.05)。结论：Levo加入STH-2停搏液能够明显减轻缺血再灌注心肌的损伤,其作用与浓度相关，机制可能与其开放ATP敏感的钾离子通道(KATP)有关。

关键词: 左西孟旦, 停搏液, 大鼠, 心肌保护, 缺血再灌注, KATP通道

Abstract: Objective:To observe the portective effect of levosimendan(Levo) in cardioplegic soIution on isoIated rat heart. Methods:Thirty-two male Wistar rats were randomly divided into four groups(n=8 in each group): no Levo(L0), 0.03μmol/L Levo(L0.03), 03μmol/L Levo(L0.3), 03μmol/L Levo and 10μmol/L glibenclamide(LG) in St.ThomasⅡ(STH-2)cardioplegia solution.The hearts was connected to Langendorff perfusion apparatus immediately.After perfused with K-H solution for 30 minutes,perfuseed by different cardioplegic solution at 25℃ for 2 hours,and then were reperfused with K-H solution for 30 minutes. Observed the changes of hemodynamics,myocardial enzyme leakage in the coronary effluent.Results: There were no significant diferences in hemodynsmics, creatine kinase（CK）and lactate dehydrogenase（LDH）before hearts stopped beating in all groups(P>0.05).After reperfusion ,the recovery of myocardial hemodynamic parameters in group L0.3 better than other groups(P<0.01 or P<0.05).LDH and CK significant dcreease in group L0.03 compared with other groups (P<0.05)..Conclusion: Levo added into STH-2 cardioplegic soIution has protective effects on myocardial isehemia-reperfusion injury，the mechanism of the protection may be related to the opening of the adenosine triphosphate-regulated potassium channel (KATP).

Key words: levosimendan, cardioplegic soIution, rats, cardioprotective effect, ischemic-reperfusion injury, KATP channel