天津医药

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胃癌组织中VEGF-D的表达与MLVD的相关性研究

邓明佳1,李科1,2,张丽娟3,沈丽达3,张灿珍 3,叶丽娟 3   

  1. 1. 云南省肿瘤医院化疗中心一病区
    2.
    3. 云南省肿瘤医院化疗研究中心一病区
  • 收稿日期:2011-01-27 修回日期:2011-08-02 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 邓明佳

Research of the level and relationship of the gastric cancer with VEGF-D and MLVD

  • Received:2011-01-27 Revised:2011-08-02 Published:2011-12-15 Online:2011-12-15

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摘要： 目的：检测胃癌患者肿瘤组织血管内皮生长因子（VEGF）-D的表达及微淋巴管密度（MLVD），分析VEGF-D与MLVD的关系。方法：收集52例胃癌术后患者标本，采用免疫组化方法测定胃癌、癌旁和正常组织MLVD及其VEGF-D的表达。结合临床及病理资料，分析VEGF-D与MLVD、年龄、性别、肿瘤组织分化、淋巴结转移以及分期等之间的关系。结果：VEGF-D在胃癌、癌旁与正常组织的阳性表达差异有统计学意义（P<0.01），而在胃癌与癌旁组织两者间的阳性表达差异无统计学意义。有淋巴结转移和Ⅲ、Ⅳ期患者胃癌及癌旁组织中VEGF-D阳性率高于无淋巴结转移和Ⅰ、Ⅱ期患者（P<0.05或P＜0.01）。癌旁MLVD高于正常组织及胃癌组织。VEGF-D阳性的癌旁组织MLVD高于VEGF-D阴性者。而VEGF-D阳性的胃癌组织MLVD与VEGF-D阴性的胃癌组织MLVD差异无统计学意义（P>0.05）。结论：胃癌癌旁组织中VEGF-D及MLVD的检测可作为评估胃癌淋巴结转移、判断胃癌生物学行为及预后的指标之一。

关键词: 胃肿瘤, 血管内皮生长因子D, 淋巴管生成, 免疫组织化学, 淋巴转移

Abstract: Objective: To detect the amount of VEGF-D (vascular endothelial growth factor-D ) and MLVD(micro-lymphatic vessel density) in the gastic cancer, analyze the relationship of VEGF-D and MLVD with the following factors: age, gender, clinical stage，lymph node metastasis，histo-differentiation and explore the use of antilymphangiogenic treatment. Methods: 52 samples of the gastric cancer tissue and bordeline tissue of cancer were gathered . 52 samples of normal tissue at least 5cm away from the tumor were also collected. We adopted an immunohistochemistry method to determine the levels of VEGF-D and MLVD in the samples. This was accomplished by bingding the clinical data and stastistical analysis to find how much VEGF-D and MLVD were present in the gastric cancer and normal tissue and the relationship with age, gender, clinical stage ，lymph node metastasis ，histo-differentiation . Results: The expressive rate of VEGF-D in gastric cancer and the normal tissue adjacent cancer had significantly difference (P<0.01 ). The positive rate of VEGF-D in gastric cancer in the samples which had lymph node metastasis was significantly higher than which had no lymph node metastasis（P<0.01 ). The positive rate of VEGF-D in gastric cancer of stage Ⅲ、Ⅳ was significantly higher than that in stage Ⅰ、Ⅱ（P<0.01）. The expression of VEGF-D had no significantly different from poor and well differentiation（P>0.05）. The expression of VEGF-D had significant correlation with lymph node metastasis ( P<0.01). The positive rate of VEGF-D was also correlated with the clinical stage（P<0.01）. The MLVD in the normal tissue was significantly higher than that in the gastric cancer ( P<0.01 ). The MLVD in tumor borderline was higher than that in the normal tissue and gastric cancer ( P<0.01). The MLVD average value in tumor borderline was significantly increased in the VEGF-D positive group than the negative group ( P<0.01). No significantly difference in the MLVD of VEGF-D positive group with the MLVD average in negative group（P>0.05）. Conclusion:The expression of VEGF-D in the gastric cancer is high, and it has correlation with clinical stage, lymph node metastasis. It suggests that the expression of VEGF-D is important in promoting tumor growth and metastasis. MLVD in the tumor borderline was significantly higher than in gastric cancer and normal tissue . The increase of MLVD may be related to the invasion and metastasis of gastric cancer in the tumor borderline. The detection of VEGF-D and MLVD in the tissue of gastric cancer can be accepted as important affiliated markers to evaluate the lymph node metastasis. It may prove to be markers to predict the malignant biology of gastric cancer and prognosis.