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ADAM17-siRNA抑制人MCF-7乳腺癌细胞体外侵袭能力的研究

杨雯静,张雪鹏,焦桂梅,赵光明,路延芹,胡宝山   

  1. 河北联合大学附属医院
  • 收稿日期:2011-02-16 修回日期:2011-05-14 出版日期:2011-11-15 发布日期:2011-11-15
  • 通讯作者: 张雪鹏

Effects of ADAM17-siRNA on the Invasion of Human MCF-7 Breast Cancer

  • Received:2011-02-16 Revised:2011-05-14 Published:2011-11-15 Online:2011-11-15

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摘要： 摘要 目的：观察人MCF-7乳腺癌细胞中解聚素-金属蛋白酶17 (ADAM17)的表达受抑制后对肿瘤细胞体外侵袭能力的影响。方法：构建可以表达针对ADAM17的小干扰RNA(siRNA)的干涉载体，转染人乳腺癌细胞系MCF-7。利用Real-time PCR法检测肿瘤细胞内ADAM17 mRNA的表达水平，Western blot 法检测ADAM17 蛋白的表达，Transwell方法检测肿瘤细胞的体外侵袭能力。结果：ADAM17 mRNA及蛋白在MCF-7细胞中呈高表达，转染特异性ADAM17-siRNA后，其表达显著被抑制，同时细胞的体外侵袭能力也明显受到抑制。结论：ADAM17及特异性的siRNA有望成为抗肿瘤侵袭治疗的新方法。

关键词: 乳腺癌, 解聚素-金属蛋白酶17, 小干涉RNA, 侵袭

Abstract: Abstract Objective: To investigate the effect of a disintegrin and metalloproteinase 17 (ADAM17)-siRNA on the invasion of human MCF-7 breast cancer cell by silence ADAM17 gene with siRNA. Methods: ADAM17-siRNA was transfected into MCF-7 breast cancer cells mediated by oligofectamine. ADAM17 mRNA and protein expressions were detected by real-time PCR and Western blot respectively, and the invasive ability of MCF-7 cells was studied by Transwell method. Results：ADAM17 mRNA and protein showed the high expression in MCF-7 cells, while it significantly decreased with ADAM17 siRNA treatment. Furthermore, the invasive ability of MCF-7 cells was markedly inhibited by ADAM17 siRNA. Conclusion：ADAM17 plays an important role in the invasion of MCF-7 breast cancer cells, and ADAM17-siRNA can inhibit the invasive ability of MCF-7 cell by blocking the expression of ADAM17.

Key words: Breast cancer, Adisintegrin and metalloprotease 17(ADAM17), Small interference RNA(siRNA), Invasion