天津医药

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孕期脂多糖刺激对新生子鼠肾素-血管紧张素系统活性的影响

郝雪琴   

  1. 河南科技大学动物科技学院
  • 收稿日期:2011-01-12 修回日期:2011-05-17 出版日期:2011-11-15 发布日期:2011-11-15
  • 通讯作者: 郝雪琴

Effect of Maternal Lipopolysaccharide Stimulation on the Activation of Renin-Angiotensin System in New born Offspring of Rats

Xue-Qin Hao   

  • Received:2011-01-12 Revised:2011-05-17 Published:2011-11-15 Online:2011-11-15
  • Contact: Xue-Qin Hao

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摘要： 摘要 目的：探讨孕期炎症刺激——脂多糖（LPS）对新生子鼠肾素-血管紧张素系统活性的影响。方法：12只SD孕鼠随机分为2组，每组6只；对照组腹腔注射无菌生理盐水 0.5 mL/只，LPS 组分别在孕期 8、10及12 d腹腔注射 LPS 0.79 mg/kg；采用Real-time PCR检测新生子鼠肾脏肾素和血管紧张素转化酶（ACE） mRNA水平，Western blotting 检测血管紧张素Ⅱ受体Ⅰ型（AT1-R）蛋白的表达，免疫组化检测血管紧张素（ANG）-II阳性细胞。结果：LPS组新生子鼠肾脏肾素mRNA低于对照组，差异有统计学意义（P＜0.01)，2组ACE mRNA表达差异无统计学意义（P＞0.05）；LPS组新生子鼠肾脏AT1-R蛋白表达量（1.535±0.100）明显高于对照组（0.561±0.089），差异有统计学意义（t=9.364，P＜0.01 )； ANG-II主要表达在近端小管上皮细胞， LPS组新生子鼠肾脏ANG-II阳性细胞数（73±4.81个）明显低于对照组（944±51.76个），差异有统计学意义（t=33.485，P＜0.001) 。结论：孕期炎症刺激抑制新生子鼠肾素-血管紧张素系统活性。

关键词: 炎症, 脂多糖, 后代, 高血压

Abstract: Effect of Maternal Lipopolysaccharide Stimulation on the Activation of Renin-Angiotensin System in New born Offspring of Rats HAO Xueqin College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China Abstract Objective：To explore the effect of maternal exposure to lipopolysaccharide (LPS) on the activation of renin-angiotensin system in new born offspring of rats. Methods： Twelve Sprague-Dawley rats were randomly divided into two groups (n=6 for each grou)，control group and LPS group. On the pregnant day 8, 10 and 12, rats of control group and LPS group were given intraperitoneally with normal saline 0.5 ml or LPS 0.79 mg/kg, respectively. The levels of renal rennin，angiotensin converting enzyme (ACE) mRNA and angiotensin Ⅱ type Ⅰ receptor（AT1-R) protein were detected by real-time PCR and Western blotting, respectively. The expression of angiotensin II in kidney was evaluated by immunohistochemical staining. Results：Compared with control group, the renin mRNA expression was significantly decreased，and the AT1-R protein expression was increased，in offspring of LPS group (P＜0.01). There was no significant difference in ACE mRNA expression between two groups (P＞0.05). The Angiotensin II-positive cells in kidney of LPS-treated offspring were lower compared with those of control group (P＜0.01). Conclusion：Maternal lipopolysaccharide stimulation suppresses renal renin-angiotensin system in new born offspring of rats.

Key words: inflammation, lipopolysaccharide, offspring, hypertension