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清醒大鼠高胰岛素-正血糖钳夹技术的建立和应用

冯悦华1,罗光华1,陈立新2   

  1. 1. 常州市第一人民医院
    2. 中国科学院上海实验动物中心
  • 收稿日期:2013-07-03 修回日期:2013-09-01 出版日期:2014-01-15 发布日期:2014-01-15
  • 通讯作者: 罗光华

Establishment and Application of Hyperinsulinemic-Euglycemic Clamp Technique in the Conscious Rats

  • Received:2013-07-03 Revised:2013-09-01 Published:2014-01-15 Online:2014-01-15

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摘要： 目的 建立大鼠高胰岛素-正血糖钳夹模型， 并探讨短期输注脂肪乳对清醒状态下大鼠葡萄糖输注率（GIR） 的影响。方法 SPF级雄性SD大鼠10只， 随机数字表法分为2组， 每组5只。采用颈动、 静脉插管技术， 分别经颈静脉给予SD大鼠输注20%脂肪乳 （脂肪乳组） 和5%葡萄糖6 h （对照组）， 行高胰岛素-正血糖钳夹实验测定血浆游离脂肪酸（FFA） 和GIR。结果 与葡萄糖输注的对照组比较， 脂肪乳组大鼠输注20%脂肪乳6 h后， 血浆FFA水平升高到对照组的17.6倍 （P < 0.01）， GIR下降了27% （P < 0.001）， 胰岛素抵抗非常明显。结论 成功建立大鼠高胰岛素-正血糖钳夹技术， 给大鼠输注脂肪乳可导致明显的胰岛素抵抗。

关键词: 高胰岛素-正血糖钳夹技术, 游离脂肪酸, 葡萄糖输注率, 胰岛素抵抗

Abstract: Objective: To establish hyperinsulinemic- euglycemic clamp technique in conscious rats, and to explore the effect of acute infusion of lipid on insulin sensitivity of rats. Methods: Rats were catheterized in the right jugular vein and left carotid artery, and underwent a hyperinsulinemic- euglycemic clamp(HEC) with infusion of lipid for 6 hours, in contrast with continuing infusion of 5% glucose. The glucose infusion rate (GIR) and plasma FFA were measured, respectively. Results: As expected, FFA concentration increased by 17.6-fold, and glucose infusion rates (GIR) was reduced by 27% in the Intralipid group compared to the controls with the 5% glucose group. Conclusion: A rat model of insulin resistance has been successfully esabished by intravenous intralipid infusion, which can be confirmed by the HEC technique.

Key words: HEC, FFA, GIR, IR