天津医药

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胰腺癌细胞中GDNF对MMP－9表达的影响

郭仁德1,王伟忠1,顾建华1,王毅2,谷川3   

  1. 1. 天津市第一中心医院普通外科
    2. 天津第一中心医院
    3. 天津市第一中心医院
  • 收稿日期:2012-02-20 修回日期:2012-09-26 出版日期:2013-01-15 发布日期:2013-01-15
  • 通讯作者: 郭仁德

Influence Of GDNF To The Expression Of Matrix Metalloproteinase 9 In Pancreatic Carcinoma

  • Received:2012-02-20 Revised:2012-09-26 Published:2013-01-15 Online:2013-01-15

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摘要： 目的探讨胰腺癌细胞中胶质细胞源神经营养因子(GDNF)对基质金属蛋白酶(MMP)-9表达的影响。方法用DMEM培养基进行人胰腺癌细胞株MIAPaCa-2和Capan-2细胞培养,利用RT-PCR、Westernblot和ELISA法研究胰腺癌细胞中GDNF对MMP-9mRNA及蛋白表达的影响。结果 RT-PCR实验发现胰腺癌细胞中GDNF能上调MMP-9mRNA的表达,Westernblot法、ELISA法检测结果表明胰腺癌细胞中GDNF能上调MMP-9蛋白的表达,且于GDNF浓度为100μg/L,作用48h时达最大值。结论 GDNF具有上调胰腺癌细胞MMP-9表达的作用,从而增强胰腺癌细胞的侵袭能力。

关键词: 胰腺癌, GDNF, MMP－9, 表达

Abstract: Abstract: Objective To investigate the influence of Glial Cell Line–Derived Neurotrophic Factor(GDNF) to the expression of Matrix Metalloproteinase 9(MMP-9) in pancreatic carcinoma. Methods Make use of RT-PCR 、Western blot and Elisa to investigate the effect of GDNF to the expression of MMP-9 mRNA and protein in MIA PaCa-2 and Capan-2 human pancreatic carcinoma cell line. Results We found that GDNF can up-regulate the expressions of MMP-9 mRNA and its protein in a dose-dependent manner in RT-PCR and Elisa experiments. Conclusions GDNF has the effect to stimulate the expression of MMP-9 and thereby strengthen the anility of invasion in panereatic carcinoma. Key Words: Pancreatic Carcinoma; GDNF; MMP-9; Expression

Key words: Pancreatic Carcinoma, GDNF, MMP-9, Expression