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N-乙酰半胱氨酸对体外循环致大鼠脑损伤的保护作用

曹惠鹃1,周锦1,张铁铮1,孙莹杰2   

  1. 1. 沈阳军区总医院
    2. 沈阳军区总医院麻醉科
  • 收稿日期:2011-05-04 修回日期:2011-09-21 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 曹惠鹃

Protective effect of N-acetylcysteine on brain injury induced by cardiopulmonary bypass in rats

  • Received:2011-05-04 Revised:2011-09-21 Published:2012-02-15 Online:2012-02-15
  • Contact: Hui-Juan CAO

摘要： N-乙酰半胱氨酸对体外循环致大鼠脑损伤的保护作用 曹惠鹃, 周锦,张铁铮,孙莹杰,姚婧 摘要 目的：探讨N-乙酰半胱氨酸（NAC）对体外循环(CPB)致大鼠脑损伤的保护作用。方法：将24只成年雄性SD大鼠随机分为3组：假手术组(S组)、CPB组(C组)、CPB+NAC组(N组)。N组在预充液中加入NAC100mg／kg，然后以20mg/（kg?h）速度输注直到停转流,C组输注等量生理盐水。停CPB后2 h，测定血浆神经元特异性烯醇化酶(NSE)、S-100β蛋白、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平，检测脑组织丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-px）含量，透射电镜下观察海马区神经元细胞超微结构的变化。结果：N组血浆NSE、S-100β蛋白、TNF-α和IL-6水平，脑组织MDA和GSH-px 含量均显著优于C组；N组海马区神经元细胞超微结构的损伤程度也较C组明显减轻。结论：NAC可减轻CPB致大鼠脑损伤，其机制可能与抗氧化、抗炎作用有关。 [关键词] N-乙酰半胱氨酸；心肺转流术；神经元特异性烯醇化酶；S-100β蛋白

关键词: N-乙酰半胱氨酸, 心肺转流术, 神经元特异性烯醇化酶, S-100β蛋白

Abstract: Protective Effect of N-acetylcysteine on Brain Injury Induced by Cardiopulmonary Bypass in Rats CAO Huijuan, ZHOU Jin , ZHANG Tie-zheng, SUN Ying-jie, YAO Jing. Department of Anesthesiology, The General Hospital of ShenYang Military Region, Shenyang 110840,China Abstract Objective :To investigate the protective effect of N-acetylcysteine (NAC) on brain injury induced by cardiopulmonary bypass (CPB) in a rat model. Methods:Twenty-four adult male Sprague-Dawley rats were randomly divided into three groups ( eight per group ) , including sham-operated control group(group S)，vehicle control group (group C), CPB plus NAC group (group N).Rats in group N were treated with NAC 100 mg／kg into CPB prime followed by infusion at 20 mg/(kg?h) until the cessation of CPB,which in group C were treated with the same volume of natural saline. The CPB model of rat was established. The animals of group C and group N underwent one hour CPB and were observed two hours later. The samples of plasma and brain tissue were collected at two hour after CPB. The plasma levels of neuron specific enolase (NSE)，S-100β protein，tumor necrosis factor -α (TNF-α),interleukin-6 (IL-6) were determined.The contents of brain tissue of malondialdehyde (MDA) and glutathione peroxidase (GSH-px) were determined.The ultrastructure of hippocampal neuron was also examined under an electron microscope. Results :The plasma levels of NSE，S-100β protein ,TNF-α,IL-6 of group N were significantly better than those of group C. The contents of brain tissue of MDA and GSH-px of group N were significantly better than those of group C. Compared with group C ,the damage degree of ultrastructure of hippocampal neuron of group N significantly reduced. Conclusion:NAC can reduce brain injury induced by CPB in a rat model by antioxidant and anti-inflammatory mechanism. Keywords N-acetylcysteine; Cardiopulmonary bypass; Neuron specific enolase ; S-100βprotein

Key words: N-acetylcysteine, Cardiopulmonary bypass, Neuron specific enolase, S-100βprotein