天津医药

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VEGF-C、VEGFR-3 和 LYVE-1 的表达在胃癌淋巴管生成及预后中的作用

刘新兰1,赵艳姣2,马志兰3   

  1. 1. 宁夏医科大学总医院肿瘤医院肿瘤内科
    2. 宁夏医科大学
    3. 宁夏医科大学2009级研究生3班
  • 收稿日期:2012-10-26 修回日期:2013-02-04 出版日期:2013-08-15 发布日期:2013-08-15
  • 通讯作者: 刘新兰

Effects of VEGF-C, VEGFR-3 and LYVE-1 on Lymphangiogenesis and Prognosis in Gastric Cancer

LIU Xinlan 1,xinlan Liu2,zhilan-Ma 2   

  1. 1. The General Hospital of Ningxia Medical University, Yinchuan 750004， China
    2. Ningxia Medical University
  • Received:2012-10-26 Revised:2013-02-04 Published:2013-08-15 Online:2013-08-15
  • Contact: LIU Xinlan

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摘要： 　【摘要】目的 检测胃癌组织中血管内皮生长因子C(VEGF-C)、血管内皮细胞生长因子受体-3(VEGFR-3)及淋巴管透明质酸受体-1(LYVE-1)的表达，探讨这3种因子在胃癌淋巴管生成和预后中的作用。方法 应用组织微阵列技术(TMA)，通过免疫组化SP法检测125例胃癌组织标本、96例癌旁正常组织及20例胃良性病变中VEGF-C、VEGFR-3和LYVE-1的表达，并对podoplanin标记微淋巴管密度(LVD)进行计数。结果 VEGF-C、VEGFR-3和LYVE-1在胃癌组织中的阳性表达率分别为62.4%、56.0%和58.4%高于癌旁正常组织中的10.4%、12.0%和9.4%及胃良性病变组织中的20.0%、30.0%和25.0%；胃癌组织中LVD表达量为2.98±0.81(癌内)/4.22±1.09(癌周)高于癌旁正常组织中1.82±0.63和胃良性病变组织的0.89±0.45(均P<0.05)，且癌周LVD计数高于癌内LVD计数(P<0.01)；三者表达与LVD均呈正相关(均P<0.01)；VEGF-C、VEGFR-3和LYVE-1表达阳性是胃癌淋巴结和远处转移的危险因素，阴性表达者5年生存明显高于阳性表达者(P<0.05)。结论 胃癌组织中存在VEGF-C、VEGFR-3和LYVE-1的高表达，是影响胃癌患者预后的危险因素，检测这3个指标可预测胃癌淋巴管生成和判断预后。

关键词: 胃肿瘤, 癌, 血管内皮生长因子C(VEGF-C), 血管内皮生长因子受体-3 (VEGFR-3), 淋巴管生成, 预后, 淋巴管透明质酸受体-1(LYVE-1), 组织微阵列技术(TMA)

Abstract: [Abstract] Objective   To study the expression of Vascular endothelial growth factor C(Vegf-C),vascular endothelial growth factor receptor-3(VegfR-3),lymphatic vessel hyaluronan receptor-1(lyve-1) in gastric cancer, To analyze the possible relationship between the expression of Vegf-C,VegfR-3 and lyve-1 with the lymphangiogenesis and prognosis. Methods  Application of Tissue Microarray Technology, The immunohistochemical technique SP was used to detect the Vegf-C, VegfR-3 and lyve-1 in 125 gastric cancer specimens and 96 normal tissues and 20 patients with benign gastric lesion．The lymphatic vessel density (LVD) was assayed by Podoplanin marks . Results    The positive rate of Vegf-C, VegfR-3 and lyve-1 in gastric cancer tissue were 62.4%,56.0% and 58.4%, higher than those in adjacent normal tissue and in gastric lesion tissue,which were 10.4%,12.5%,9.4% and 20%,30%,25% (P<0.05).The LVD score in gastric cancer was significantly higher than in adjacent normal tissues or benign gastric lesion (P<0.01)(2.98±0.81/4.22±1.09vs1.82±0.63or0.89±0.45),the LVD score in peri-tumoral was higher than in intra-tumoral(P<0.01).There is a positive relationship between expression of Vegf-C,VegfR-3 and lyve-1 with LVD (P<0.05). The positive expression of three indexes were the risk factors of lymph node metastasis and distant metastasis(P<0.05). The negative expression of three indexes also had a long 5 year survival rates(P<0.05). Conclusion   Vegf-C, VegfR-3 and lyve-1 protein positively highly expressed in gastric cancer tissue,which were risk factors influence the prognosis of gastric cancer.The positive expression of three indexes can be used to predict the prognosis and lymphangiogenesis of gastric cancer.

Key words: stomach neoplasms, carcinoma, Vegf-C, VegfR-3, angiogenesis, prognosis, lyve-1, 组织微阵列技术(TMA)