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乌司他丁对大鼠重症急性胰腺炎肺损伤中SP-A的影响研究

李楠   

  1. 天津医科大学总医院
  • 收稿日期:2012-05-09 修回日期:2012-10-12 出版日期:2013-03-15 发布日期:2013-03-15
  • 通讯作者: 李楠

The study on the mechanism of Ulinastatin for treatment of lung injury induced by severe acute pancreatitis in rat

LI Nan   

  1. Department of General Surgery, General Hospital of Tianjin Medical University, Tianjin 300052, China
  • Received:2012-05-09 Revised:2012-10-12 Published:2013-03-15 Online:2013-03-15
  • Contact: LI Nan

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摘要：

【摘要】目的 观察乌司他丁对重症急性胰腺炎（SAP）肺损伤大鼠的肺泡表面活性物质相关蛋白A（SP-A）含量的影响，探讨其对SAP肺脏损伤的保护机制。方法 SD大鼠60只，随机分为空白对照组、SAP模型组、乌司他丁组 3组，每组20只。通过胆胰管内逆行注入5%牛磺胆酸钠法建立大鼠SAP 致急性肺损伤模型，用ELISA法检测支气管肺泡灌洗液(BALF)中SP-A的含量，离心灌洗液行细胞分类计数，测量肺湿/干重比以判断肺水肿情况。结果 SAP组大鼠肺湿/干重比值、支气管肺泡灌洗液中的中性粒细胞含量高于空白对照组和乌司他丁治疗组，乌司他丁组大鼠肺湿/干重比值和中性粒细胞含量高于空白对照组(P < 0.05）；SAP组支气管肺泡灌洗液中SP-A含量和巨噬细胞低于空白对照组和乌司他丁组，乌司他丁组低于对照组，SAP组和乌司他丁组淋巴细胞高于空白对照组(P < 0.05)；3组大鼠肺泡灌洗液中总细胞数差异无统计学意义。结论  SAP肺损伤时肺泡内SP-A含量明显减少，乌司他丁对SAP时肺泡内SP-A起保护作用。

关键词: 重症急性胰腺炎, 急性肺损伤, 肺泡表面活性物质相关蛋白A, 乌司他丁

Abstract:

[Abstract] Objective  To evaluate the effect of ulinastatin on the level of surfactant protein A (SP-A) in bronchoalveolar lavage fluid (BALF) in lung injury induced by severe acute pancreatitis (SAP) in rat model, and protection mechanisms of ulinastatin thereof. Methods Sixty SD rats were randomly divided into 3 groups: control group (n=20), SAP model group (n=20) and ulinastatin group (n=20). The SAP model was induced by retrograde infusion of 5% sodium cholate into the bile-pancreatic duct. Twenty-four hour after operation, the left lung’s BALF liquid was collected and the level of SP-A in BALF liquid was detected by ELISA method. The BALF liquid was differentially counted by using microscope. The wet-to-dry lung weight ratio was recorded. Results The value of wet/dry lung weight ratio and the level of neutrophil granulocytes in BALF were higher in SAP group than those of control group and ulinastatin group. The wet/dry lung weight ratio and the neutrophil granulocyte content in BALF were significantly higher in ulinastatin group than those of control group (P < 0.05). The levels of SP-A and macrophages in BALF were significantly lower in SAP group than those of control group. The level of lymphocytes was significantly higher in SAP group and ulinastatin group than that of control group (P < 0.05). There were no significant differences in total cell number of BALF between three groups. Conclusion The level of SP-A in BALF was lower in lung injury induced by SAP. Ulinastatin can protect the SP-A of BALF in SAP rats.

Key words: severe acute pancreatitis, acute lung injury, surfactant protein A, Ulinastatin