天津医药

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西维来司钠对大鼠重症急性胰腺炎后急性肝损伤的保护作用

赵宝川1,彭彦辉2,脱红芳2,耿晓峰2   

  1. 1. 河北省保定市高阳县县医院
    2. 河北省人民医院
  • 收稿日期:2010-07-14 修回日期:2010-10-14 出版日期:2011-05-15 发布日期:2011-05-15
  • 通讯作者: 彭彦辉

The Effect of Sivelestat against pancreatic injury after severe acute pancreatitis in rats

  • Received:2010-07-14 Revised:2010-10-14 Published:2011-05-15 Online:2011-05-15

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2008

摘要： 目的：探讨西维来司钠对大鼠重症急性胰腺炎后急性肝损伤的治疗价值。方法：SD大鼠54只,随机分为3组,A组为对照组(n=18)，B组为重症急性胰腺炎(SAP)模型组(n= 18)，C组为西维来司钠药物治疗组(n=18)。3.5%牛磺胆酸钠逆行胰胆管内注射诱发大鼠SAP动物模型,于术后3h、6h、12h检测血浆IL-6、NE、ALT及肝组织的SOD、MDA、MPO并观察肝、胰病理变化。结果:①SAP组血浆IL-6、NE、AMY、ALT明显升高(P<0.05),镜下可见胰腺水肿、炎细胞浸润、坏死,肝脏肝窦充血、细胞浊肿及坏死,且损伤程度随时限延长而加重;②SAP各组MDA、MPO明显升高(P<0.05)、SOD明显降低(P<0.05)。③西维来司钠治疗组与SAP组比较血浆IL-6、NE、MDA、MPO水平明显下降(P<0.05),SOD明显升高(P<0.05),胰、肝病理损害程度减轻,并且AMY、ALT均明显降低(P<0.05)。结论:西维来司钠通过抑制中性粒细胞弹性蛋白酶的活性及在肝组织中的表达，抑制SAP急性期炎症反应及有效提高机体对氧自由基的清除能力，减轻SAP中肝损伤的程度。

关键词: 重症急性胰腺炎, 肝损伤, 氧自由基, IL-6, NE, 西维来司钠

Abstract: Objectives：To study the role of Sivelestat in the hepatic injury in rats with severe acute pancreatitis. Methods：Fifty-four SD rats were randomly divided into three groups: Control group (group A), severe acute pancreatitis group (group B) and treatment group with Sivelestat (group C). Each group was divided into 3h, 6h and 12h subgroups (n=6 in each subgroup). SAP was induced by retrograde injection of 3.5% sodium taurocholate into biliopancreatic duct. The level of serum IL-6, NE and ALT were measured. The levels of SOD, MDA ,MPOin hepatic tissue bomogenate were measured. Part of hepatic and pancreatic tissue were changed by HE staining. Results: ①In the SAP group serum levels of IL-6, NE, AMY, ALT increased significantly(P<0.05)and the pancreas and liver showed obvious pathologic changes. ②In the SAP group, the level of liver tissue bomogenate MDA and MPO increased significantly, and SOD significantly reduced. ③The treatment with Sivelestat, the levels of IL-6, NE, ALT, MDA and MPO decreased significantly and that of SOD increased, the liver and pancreatic pathologic damages score improved. Conclusion: The treatment with Sivelestat, a elastase inhibitor, could markedly atteneuate hepatic injury in SAP by effectively enhance the oxygen free radical scavenging capacity and inhibit neutrophil elastase and inflammatory reaction.

Key words: severe acute pancreatitis (SAP), liver damage(LD), oxygen free radical(OFR), interleukin-6(IL-6), neutrophil elastase(NE), Sivelestat