天津医药

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YD383和YD439对IL-4诱导的BJAB细胞CD23表达的影响

笪宇蓉1,邢军2,段宏泉3,董学易4,杨洁5   

  1. 1. 天津医科大学
    2. 天津医科大学附属口腔医院
    3. 天津医科大学药理教研室
    4. 天津医科大学基础研究中心
    5. 天津医科大学免疫教研室
  • 收稿日期:2010-07-22 修回日期:2010-11-17 出版日期:2011-03-15 发布日期:2011-03-15
  • 通讯作者: 笪宇蓉

The Effect of YD383 and YD439 on the Expression of CD23 Induced by IL-4 in BJAB Cells

  • Received:2010-07-22 Revised:2010-11-17 Published:2011-03-15 Online:2011-03-15
  • Contact: rong yu DA

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摘要： 摘要 目的：观察两种小分子化合物YD383，YD439 对IL-4诱导的BJAB 细胞 CD23分子表达的影响，初步探讨两种化合物对JAK/STAT6信号传导通路的抑制机理。方法：将不同剂量的化合物YD383和YD439加入对数生长期的BJAB细胞作用半小时，终浓度为20ng/mL 的IL-4共同孵育3小时后，收集细胞并加入PE标记的鼠抗人CD23抗体，流式细胞仪检测各细胞群体CD23分子的表达情况。结果：化合物YD383和YD439均能降低由IL-4诱导的BJAB细胞CD23分子的表达，且随着化合物剂量的增大，CD23分子的表达水平逐渐降低,。两种化合物各自不同的剂量组间，CD23分子表达 的差异均有统计学意义（P<0.05）。结论：化合物YD383和YD439对IL-4诱导的CD23分子的表达均有明显的抑制作用，并且具有剂量依赖性。

关键词: CD23, IL-4, JAK, /STAT6, 细胞因子

Abstract: Abstract Objective: To examine the effect of compounds YD383 and YD439 on the expression of CD23 induced by IL-4 in BJAB cells. Methods: The BJAB cells of exponential growing were treated with YD383 or YD439 in different dose for 30 minutes. Then cells were incubated with IL-4 (20ng/ml) for 3 h. Cells were collected and mixed with PE-lableled CD23 antibody. The expression of CD23 in different conditions was detected by Flow Cytometry. Results: The expression of CD23 induced by IL-4 in BJAB cells were significantly inhibited by YD383 and YD439 in a dose dependent manner （P<0.05） Conclusion: YD383 and YD439 can inhibit the expression of CD23 induced by IL-4 in BJAB cells. The expression of CD23 decreased in time-dependent manner.

Key words: CD23, IL-4, JAK-STAT6 cytokine