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咖啡酸苯乙酯对糖尿病大鼠视网膜VEGF和ICAM-1表达的影响

袁鹏1,臧婵媛1,周伟2,刘学政3   

  1. 1. 天津医学高等专科学校
    2. 天津医大二院
    3. 辽宁医学院
  • 收稿日期:2010-06-10 修回日期:2011-01-25 出版日期:2011-06-15 发布日期:2011-06-15
  • 通讯作者: 袁鹏

The effects of caffeic acid phenethyl ester on the expression of VEGF and ICAM-1 in the retinae of diabetic rats

  • Received:2010-06-10 Revised:2011-01-25 Published:2011-06-15 Online:2011-06-15

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摘要： 【摘要】 目的 观察咖啡酸苯乙酯对糖尿病大鼠视网膜血管内皮生长因子（VEGF ）和细胞间黏附因子-1（ICAM-1）蛋白表达的影响。方法 将30只STZ诱导的糖尿病大鼠随机分为3组，糖尿病组（DM，n=10），CAPE低剂量治疗组（CAPE5，n=10），CAPE高剂量治疗组（CAPE10，n=10）。另取10只大鼠作为正常对照组（CON，n=10）。饲养三个月后，取大鼠双眼视网膜做组织匀浆，Elisa法检测视网膜VEGF 和ICAM-1的表达。结果 腹腔注射咖啡酸苯乙酯（CAPE）后，糖尿病大鼠视网膜VEGF和ICAM-1蛋白水平表达降低，低剂量治疗组可分别减少40%VEGF和29% ICAM-1蛋白的表达，高剂量治疗组可分别减少42%VEGF和44% ICAM-1蛋白的表达。 结论 咖啡酸苯乙酯可减少糖尿病大鼠视网膜VEGF 和ICAM-1蛋白的表达。

关键词: 糖尿病视网膜病变, 咖啡酸苯乙酯, 血管内皮生长因子, 细胞间黏附因子-1

Abstract: [Abstract] Objective To observe the effccts of caffeic acid phenethyl ester on the expression of VEGF and ICAM-1 in the retinae of diabetic rats. Method Thirty diabetic SD rats Inducided by streptozotocin were divided into three groups, diabetic group (DM, n=10) ,low dose group of caffeic acid phenethyl ester（CAPE5，n=10）. High dose group of caffeic acid phenethyl ester（CAPE10，n=10）.Ten else rats were in normal control group.All of the rats were executed 3 months later , both side of the retinae were homogenized in each rat, and the protein level of VEGF and ICAM-1 was detected by Elisa. Results After peritoneal injection of caffeic acid phenethyl ester ,the protein level of VEGF and ICAM-1 were decreased ,in the low dose group, the protein level of VEGF and ICAM-1 were decreased by 40% and 29%,when the protein level of VEGF and ICAM-1 were decreased by 42% and 44% in high dose group. Conclusion caffeic acid phenethyl ester can reduce the expression of VEGF and ICAM-1 in retinae of diabetic rats.

Key words: Diabetic retinopathy, Caffeic acid phenethyl ester, VEGF, ICAM-1