天津医药

• 实验研究 •    

TGF-β1、CTGF基因的过表达与早期糖尿病肾病关系的研究

于倩1,刘德敏2,张捷1,3   

  1. 1. 天津医科大学代谢病医院
    2. 医科大学代谢病医院
    3.
  • 收稿日期:2011-05-19 修回日期:2011-06-14 出版日期:2012-03-15 发布日期:2012-03-15
  • 通讯作者: 于倩

Association of TGF-β1 and CTGF with Early Diabetic Nephropathy

  • Received:2011-05-19 Revised:2011-06-14 Published:2012-03-15 Online:2012-03-15
  • Contact: Qian Yu

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摘要： 摘要 目的：探讨肾脏纤维化的关键细胞因子转化生长因子-β1(TGF-β1)、结缔组织生长因子（CTGF）在早期糖尿病肾病（DN）中的作用。 方法：建立链脲佐菌素（STZ）诱导的糖尿病动物模型，随机分为正常对照组（NC）和糖尿病组（DM）。每周测量体质量，每2周测定血糖，于实验第8周末处死大鼠后测定相关的生化指标及肾功能指标，同时留取肾皮质液氮冻存，应用实时定量PCR方法检测肾皮质TGF-β1、CTGF的mRNA水平，用免疫组化法检测肾小球中TGF-β1和CTGF的蛋白表达情况，并进行半定量分析。结果: 与NC组相比，DM组血糖、血尿素氮、24 h尿量及尿微量蛋白、肾脏肥大指数明显升高（P＜0.01），但血肌酐明显下降（P＜0.01）。实时定量PCR及免疫组化结果显示，DM组较NC组TGF-β1、CTGF mRNA及蛋白表达量均明显增高。结论: TGF-β1与CTGF在肾病早期表达明显升高，对于评价DN进程具有重要意义。

关键词: 糖尿病肾病, 转化生长因子β1, 结缔组织生长因子

Abstract: Abstract Objective:To investigate the effect of Transfoming growth factor-β1（TGF-β1） and Connective tissue growth factor（CTGF）in early stage of diabetic nephropathy. Methods:Murine diabetic models were induced with streptozotocin (STZ). We randomized rats into normal control group (NC) and diabetes group (DM). Measure the body weight every week, and the blood glucose every two weeks. All the rats were sacrificed after 8 weeks, a set of biochemical data and indicators related to renal function were detected in each group. At the same time, the liquid nitrogen frozen specimens from renal cortex were taken to use to analyze the level of TGF-β1、CTGF mRNA by the method of real time RT-PCR. The protein expression of TGF-β1 and CTGF were determined by immunohistochemistry. Results:Compared with NC group, the level of blood glucose, BUN, 24h urine and urinary microalbumin, KW/BW was significantly higher in DM group（P＜0.01）, but the level of Scr was significantly lower（P＜0.01）. The results of Real time quantitative PCR showed obvious increase of TGF-β1 and CTGF mRNA in DM group，as well as their protein expression, and the level of CTGF increased more significantly（P＜0.01）. Conclusion:The level of TGF-β1 and CTGF mRNA and expression of protein was significantly increased in diabetic nephropathy （DN），they are very important for evaluating the process of DN.

Key words: diabetic nephropathies, transforming growth factor beta1, connective tissue growth factor