天津医药

• 实验研究 •    

冬凌草甲素抑制体内外卵巢癌生长的作用

胡瑞华   

  1. 湖北省妇幼保健院徐东门诊部妇科
  • 收稿日期:2011-05-11 修回日期:2011-10-18 出版日期:2012-03-15 发布日期:2012-03-15
  • 通讯作者: 胡瑞华

The anti-tumor effect of oridonin on ovarian cancer in vitro and in vivo

  • Received:2011-05-11 Revised:2011-10-18 Published:2012-03-15 Online:2012-03-15

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摘要： 【摘要】目的 探讨冬凌草甲素对体内外卵巢癌生长的影响及其机制。方法 冬凌草甲素作用人卵巢癌细胞株HO-8910PM后，MTT法检测细胞增殖；流式细胞术检测细胞凋亡；Western blotting检测卵巢癌细胞中NF-kB和XIAP蛋白表达；建立起裸鼠卵巢癌皮下移植瘤模型，观察冬凌草甲素对裸鼠卵巢癌皮下移植瘤生长的影响；免疫组织化学法检测肿瘤组织中Ki-67、NF-kB和XIAP的阳性表达。结果 与对照组相比较，冬凌草甲素可显著抑制体外卵巢癌HO-8910PM细胞生长，并明显诱导细胞凋亡；冬凌草甲素可显著下调HO-8910PM细胞中NF-kB和XIAP的表达；冬凌草甲素可显著抑制裸鼠卵巢癌皮下移植瘤生长，同时明显降低皮下移植瘤组织中Ki-67、NF-kB和XIAP的阳性表达。结论 冬凌草甲素可显著抑制体内外卵巢癌生长，该作用可能通过抑制NF-kB及其调控蛋白XIAP的表达而实现。

关键词: 卵巢癌, 冬凌草甲素, NF-kB

Abstract: 【Abstract】 Objective To investigate the effect and the mechanism of oridonin in the growth inhibition of ovarian cancer. Methods After human ovarian cancer cell line HO-8910PM was treated with different concentrations of oridonin, the cellular proliferation was detected by MTT assay. The flow cytometry was used to determine apoptosis in ovarian cancer cells. Western blot was used to detect the expression of NF-kB and XIAP in ovarian cancer cells. Furthermore, HO-8910PM cells were injected subcutaneously into nude mice to establish xenograft model. After 2 weeks of implantation, mice were randomized into 2 groups (n = 10): Control group, feed with 0.1% Dmethyl sulfoxide (DMSO) 0.2 ml; Test group, feed with oridonin at a dose of 40 mg/kgbw. All treatment lasted for three weeks, thrice per week. Six weeks after implantation, tumor weight and inhibition rate were evaluated respectively after the mice were sacrificed. Immunohistochemistry was used to detect the positive expression of Ki-67, NF-kB and XIAP in the tumors. Results The proliferation of ovarian cancer cells was inhibited significantly by oridonin. Apoptosis rate induced by the oridonin was markedly highter than that of control. The expression of NF-kB and XIAP were down-regulated in HO-8910PM cells after treatment of oridonin. The final tumor weight showed significant decrease in the test group compared with control. Furthermore, the positive expression of Ki-67, NF-kB and XIAP were decreased in tumors after administration of oridonin. Conclusion Oridonin exerts anti-tumor activity in ovarian cancer both in vitro and in vivo, which may be related to down-regulation of NF-kB and its regulated molecules such as XIAP.

Key words: ovarian cancer, oridonin, NF-kB