天津医药

• 细胞与分子生物学 • 上一篇    下一篇

纤维支气管镜活检组织Sox2基因的检测对肺癌的诊断价值

李劳冬,莫碧文,于会娜,王昌明,曾锦荣,王绩英,韦江红,黄剑伟   

  1. 桂林医学院附属医院
  • 收稿日期:2013-06-19 修回日期:2013-10-27 出版日期:2014-04-15 发布日期:2014-04-15
  • 通讯作者: 莫碧文

The Diagnostic Value of Sox2mRNA Transcription Level in Bronchoscopy Biopsy Specimens in Lung Cancer

  • Received:2013-06-19 Revised:2013-10-27 Published:2014-04-15 Online:2014-04-15

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摘要：

【摘要】 目的 探讨纤维支气管镜活检组织标本中干细胞标志物Sox2 mRNA对肺癌的诊断价值。方法 采用 RT-PCR技术检测100例肺癌患者和18例良性肺疾病患者纤维支气管镜活检标本中Sox2 mRNA的表达， 随后利用免疫组化技术验证Sox2蛋白在50例肺癌组织、 32例良性病变肺组织和18例癌旁正常肺组织间表达的差异， 分析Sox2 mRNA表达与肺癌临床病理参数的关系及其在肺癌诊断中的潜在价值。结果 Sox2 mRNA和蛋白在肺癌组织中的表达量显著高于非癌肺组织 （P＜0.05）。肺癌患者不同年龄、 性别、 病理类型、 TNM分期、 肿瘤分化程度及淋巴结是否转移， Sox2 mRNA的表达差异无统计学意义。Sox2 mRNA ROC曲线下面积为0.748， 临界值取0.513时， 其灵敏度和特异度分别为85.0%和61.1%。结论 纤维支气管镜活检组织标本Sox2 mRNA可能是肺癌诊断的有效标志物。

关键词: 肺癌, Sox2, 干细胞标志物, 诊断标志物, 纤维支气管镜

Abstract:

[Abstract] Objective To study the diagnostic value of Sox2 mRNA transcription level in bronchoscopic biopsy specimens from lung cancer patients. Methods The expression of Sox2 mRNA was detected using RT-PCR from 100 hu? man lung cancer biopsy and 18 non-cancer lung biopsy through bronchoscopy. The expression of Sox2 protein was examined by immunohistochemistry from 50 cases of lung cancer biopsy, 32 cases of benign lung lesions and 18 cases of pericarcino? matous normal lung tissues. Then the relationships between Sox2 mRNA transcription level and lung cancer clinical patho? logical parameters were analyzed to test the diagnostic value of Sox2 transcription level. Results The transcription of Sox2 mRNA and its protein expression level were significantly higher in lung cancer than that in benign pulmonary disease tissues （P＜0.05） . The transcription of Sox2 mRNA was not correlated with age, gender, histology, lymph node metastasis, TNM stage and differentiation of lung cancer patients (P＞0.05). The Sox2 mRNA yielded an area of 0.748 under the ROC curve with the sensitivity of 85.0% and the specificity of 61.1%, taking the cut-off value of 0.513. Conclusion The Sox2 mRNA might be a useful diagnostic marker for lung cancer.

Key words: lung cancer, Sox2, stem cells marker, diagnostic marker, bronchoscope