天津医药

• 实验研究 • 上一篇    下一篇

枸杞多糖抑制对硫磷致血管内皮功能损伤的研究

李鹏,尹雅玲,卢光洲,赵繁荣,梁金英   

  1. 新乡医学院
  • 收稿日期:2010-05-20 修回日期:2010-09-06 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 李鹏

Study of Inhibition Effect of LBP on Blood Vessel Endothelium Function Damnification induced by DNTP

  • Received:2010-05-20 Revised:2010-09-06 Published:2011-01-15 Online:2011-01-15

PDF (PC)

2332

摘要： 目的：探讨枸杞多糖（LBP）抑制对硫磷（DNTP）所致的血管内皮功能损伤。方法：DNHP与大鼠离体胸主动脉血管环（EVAPVR）和培养的人脐静脉内皮细胞（HUVEC）共孵30min，以LBP为保护、以超氧化物歧化酶（SOD）为对照，检测血管内皮依赖性舒张反应（EDRR）、内皮细胞单层通透性（ECMP）及细胞培养液生化指标。结果：血管环实验结果显示，LBP（10mg/mL）明显减轻了DNTP（4.5μmol/L）对血管EDRR的抑制作用；细胞实验结果显示，LBP（10mg/mL）明显降低了ECMP，增加了细胞培养液中SOD和一氧化氮（NO）的含量、降低了丙二醛（MDA）的含量（均P＜0.05），与对照组SOD的作用相当（均P＞0.05）。结论：LBP抑制DNTP所致的血管内皮功能损伤，其机制可能与氧化应激有关。

关键词: 枸杞, 多糖类, 对硫磷, 主动脉, 胸, 内皮细胞, 脐静脉, 血管舒张

Abstract: Objective: To explore the mechanism of inhibition effect of lycium barbarum polysaccharide (LBP) on blood vessel endothelium function damnification induced by DNTP. Method: The ex vivo aorta pectoralis vascular ring (EVAPVR) of rats and cultured human umbilical vein endothelial cells (HUVEC) were incubated with DNTP for 30 min, LBP was used as protection and superoxide dismutase (SOD) was used as control. The endothelial-dependent relaxation reaction (EDRR), endothelial cell monolayer permeability (ECMP) and biochemical index were detected. Results: Vascular circle experimental result showed that LBP (10mg/mL) obviously reduced the inhibition of ACh-induced EDRR induced by DNTP(4.5μmol/L); Cell experimental result showed that LBP (10mg/mL) obviously reduced the ECMP and MDA, timproved the SOD and nitric oxide(NO) (P＜0.05, respectively), the effect was corresponded on the SOD control(P＞0.05, respectively).Conclusion: LBP inhibited the blood vessel endothelium function damnifivation induced by DNHP,which may be related to the oxidative stress.

Key words: LYCIUM BARBARUM, polysaccharides, parathion, aorta, thoracic, endothelial cells, umbilical veins, 血管舒张