天津医药

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强直性脊柱炎患者Th淋巴细胞功能的研究

刘秀婵1,王俊祥2,魏平2   

  1. 1. 天津市天津医院
    2. 河北医科大学第三临床医院
  • 收稿日期:2010-08-02 修回日期:2010-10-18 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 魏平

The function of helper T lymphocytes in ankylosing spondylitis

  • Received:2010-08-02 Revised:2010-10-18 Published:2010-12-15 Online:2010-12-15

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摘要： 目的:探讨辅助性T细胞(Th)Th1/Th2比例失衡及Th1/Th2细胞因子网络的异常在强直性脊柱炎(AS)发病机制中的作用。方法:利用酶联免疫吸附试验(ELISA)测定30例AS伴膝关节积液患者、20例正常对照血清及AS患者关节液中Th1/Th2型细胞因子肿瘤坏死因子-α(TNF-α)和白细胞介素(IL)-10;应用三色流式细胞分析法(FCM)检测外周血Th细胞(CD3+CD4+)比例及其胞浆内Th1/Th2细胞因子干扰素-γ(IFN-γ)和IL-4的表达;AS患者同时检测红细胞沉降率(ESR)及C-反应蛋白(CRP)。结果:(1)AS患者血清TNF-α和IL-10水平均明显高于正常对照组(P<0.01),AS患者关节液TNF-α水平高于血清水平(P<0.01),IL-10水平与血清中水平差异无统计学意义(P>0.05)。(2)AS患者外周血Th、Th2细胞(CD3+CD4+IL-4+)百分率与对照组差异无统计学意义(P>0.05),而Th1细胞(CD3+CD4+IFN-γ+)百分率及Th1/Th2比值高于对照组(P<0.01)。(3)AS患者血清中TNF-α的水平与ESR、CRP呈正相关(P<0.01或P<0.05);而IL-10水平、外周血Th1和Th2细胞百分率、Th1/Th2比值与ESR、CRP均无明显相关性(P>0.05)。结论:AS患者外周血存在Th1/Th2比例失衡,为Th1优势状态。Th1型细胞因子TNF-α在AS患者外周血及关节液中均呈现高水平表达。 

关键词: 脊柱炎, 强直性, Th1细胞, Th2细胞, 细胞因子, 三色流式细胞分析法

Abstract: [Abstract] Objective To study the role of the imbalance of Th1/Th2 and Th1/Th2 cytokines in the pathogenesis of AS.Methods 30 subjects patients with AS and and 20 subjects normal controls had been chosen. Enzyme linked immunosorbent assay (ELISA) was used to measure the levels of TNF-α and IL-10 in the serum and synovial fluid. Three-color flow cytometric analysis was employed for surface antigens (CD3, CD4) of Th cells and intracellular cytokines (IFN-γ/IL-4) of Th1/Th2 in the peripheral blood of patients with AS and normal controls.Result The TNF-α and IL-10 serum levels in patients with AS were all significantly higher than in normal controls(P<0.05); The synovial fluid level of TNF-α was significantly higher than the serum level (P<0.05), The synovial fluid level of IL-10 was not significantly different from the serum level (P>0.05); The percentage of Th1 and Th2 cells in the peripheral blood of patients with AS were not significantly different from normal controls (P>0.05),The percentage of Th1 cells in the peripheral blood of patients with AS was significantly higher than normal controls, The rate of Th1/Th2 was significantly higher than in normal controls (P<0.05)；The serumlevel of TNF-α correlated significiently with ESR, CRP. There wasn’t significant correlation between the serum level of IL-10、the percentage of Th1 cells (or Th2 cells) 、 the rate of Th1/Th2 and ESR, CRP (P>0.05). Conclusion There is an imbalance in Th1/Th2 in the peripheral blood of patients with AS, and towards Th1.

Key words: Spondylitis, ankylosing, Th1 cells, Th2 cells, cytokines, three-color flow cytometric analysis