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三七有效成分对免疫性肝损伤的保护作用

韦维,李韬   

  1. 右江民族医学院
  • 收稿日期:2014-01-14 修回日期:2014-05-15 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 韦维

Protective Effects of Active Ingredients of Notoginseng on Immunological Liver Injury

  • Received:2014-01-14 Revised:2014-05-15 Published:2014-09-15 Online:2014-09-15
  • Contact: WEI Wei

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摘要：

【摘要】目的 研究三七有效成分三七总皂苷、总黄酮及多糖对小鼠免疫性肝损伤的保护作用。方法昆明种小鼠60只随机分为正常组（A组）、模型组（B组）、三七总皂苷组（C组）、三七总黄酮组（D组）、三七多糖组（E 组）。C、D、E组分别给予三七总皂苷、三七总黄酮、三七多糖灌胃，1次/d，14d后B、C、D和E组于尾静脉注射卡介苗，26d于尾静脉注射脂多糖，禁食12h后于眼球静脉丛采血，检测血清中丙氨酸转氨酶（ALT）、总超氧化物歧化酶（T-SOD）、白细胞介素-4（IL-4）的含量；取肝、脾、胸腺计算脏器指数；另取肝脏做病理学检查。结果与B组比较， C、D、E组小鼠肝、脾、胸腺指数、血清ALT水平明显降低（P<0.01），IL-4、T-SOD显著升高（P<0.01）；病理学示C、 D、E组肝脏炎症细胞浸润、水肿和点状、片状坏死程度均比B组明显减轻。结论三七总皂苷、总黄酮及多糖对免疫性肝损伤均有明显的保护作用。

关键词: 三七有效成分, 免疫性肝损伤, 卡介苗, 脂多糖

Abstract:

[Abstract] Objective To investigate the protective effects ofactive components of notoginseng on immunological liver injury in mice. Methods Sixty Kunming mice were randomly divided into normal group (group A), model group (Group B), PNS group (Group C), total flavonoids group (Group D),and polysaccharidegroup (group E). C,D and E groups were given PNS,total flavonoids and polysaccharideorally,1/day for 14days. Then BCG was intravenous injected in B, C, D and E groups, and after26days lipopolysaccharide (LPS) was intravenous injected. Samples of eye venous plexus blood were collected, and serum levels of alanine aminotransferase (ALT), total superoxide dismutase (T-SOD) and Interleukin-4(IL-4) were detected. Organs index was calculated by checking pathological results of liver, spleen and thymus.Results Com? pared with group B, the thymus index and serum ALT levels were significantly decreased in C, D and E groups (P<0.01), but the levels of IL-4and T-SOD increased significantly (P<0.01). Pathological results showed that there were more serious inflammatory cell infiltration in liver, edema and necrosis of dot in C, D and E groups than those in group B. Conclusion The active components of notoginseng showed a significant protective effect on immunological liver injury

Key words: The active components of notoginseng, Immune liver injury, BCG, LPS